DETERMINATION OF FATTY ACIDS IN THREE NITRARIA SPECIES BY PRECOLUMN FLUORESCENCE LABELING FOR HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION-MASS SPECTROMETRY

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	DETERMINATION OF FATTY ACIDS IN THREE NITRARIA SPECIES BY PRECOLUMN FLUORESCENCE LABELING FOR HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION-MASS SPECTROMETRY
	Hu, Na 1,2,3; Zhang, Shijuan 1,3; Ji, Zhongyin 1,3; You, Jinmao 1; Suo, Yourui 1,2
	2014
发表期刊	ANALYTICAL LETTERS
ISSN	0003-2719
卷号	47 期号:15 页码:2475-2487
文章类型	Article
摘要	A novel fluorescence method using 2-(7-methyl-1H-pyrazolo-[3,4-b] quinoline-1-yl) ethyl-4-methyl benzenesulfonate as the labeling reagent was established for high-performance liquid chromatography determination of fatty acids. The conditions were optimized, including the identity of the organic solvent, identity, and amount of catalyst, amount of derivatization reagent, derivatization temperature, and derivatization time. The results indicated that quantitative yields of derivative were obtained with a five-fold molar excess of reagent at 90 degrees C for 30 min using 25 mg of potassium carbonate as the catalyst. Atmospheric pressure chemical ionization mass spectrometry results indicated that collision-induced dissociation of protonated fatty acid derivatives produced fragments at m/z 228.2, m/z 210.2, and m/z 183.8. The method was validated in terms of linearity, limits of detection, limits of quantification, precision, and accuracy, and the results showed that the method exhibited excellent sensitivity, selectivity, and reproducibility. Limits of detection and limits of quantification were in the ranges of 0.52-2.34 ng mL(-1) and 1.23-6.63 ng mL(-1), respectively. This method was successfully applied to the determination of fatty acids in sarcocarps, seeds, and leaves of Nitraria tangutorum Bobr., Nitraria sibirica Pall., and Nitraria roborowskii Kom. The results indicated that the main components were oleic, linoleic, linolenic, and hexadecanoic acids. However, the composition of fatty acids in the tissues varied considerably.; A novel fluorescence method using 2-(7-methyl-1H-pyrazolo-[3,4-b] quinoline-1-yl) ethyl-4-methyl benzenesulfonate as the labeling reagent was established for high-performance liquid chromatography determination of fatty acids. The conditions were optimized, including the identity of the organic solvent, identity, and amount of catalyst, amount of derivatization reagent, derivatization temperature, and derivatization time. The results indicated that quantitative yields of derivative were obtained with a five-fold molar excess of reagent at 90 degrees C for 30 min using 25 mg of potassium carbonate as the catalyst. Atmospheric pressure chemical ionization mass spectrometry results indicated that collision-induced dissociation of protonated fatty acid derivatives produced fragments at m/z 228.2, m/z 210.2, and m/z 183.8. The method was validated in terms of linearity, limits of detection, limits of quantification, precision, and accuracy, and the results showed that the method exhibited excellent sensitivity, selectivity, and reproducibility. Limits of detection and limits of quantification were in the ranges of 0.52-2.34 ng mL(-1) and 1.23-6.63 ng mL(-1), respectively. This method was successfully applied to the determination of fatty acids in sarcocarps, seeds, and leaves of Nitraria tangutorum Bobr., Nitraria sibirica Pall., and Nitraria roborowskii Kom. The results indicated that the main components were oleic, linoleic, linolenic, and hexadecanoic acids. However, the composition of fatty acids in the tissues varied considerably.
关键词	Atmospheric Pressure Chemical Ionization-mass Spectrometry Fatty Acids High-performance Liquid Chromatography Nitraria Species Precolumn Fluorescence Derivatization
WOS标题词	Science & Technology ; Physical Sciences
关键词[WOS]	GAS-CHROMATOGRAPHY ; GC-MS ; EXTRACTION ; DISEASE ; ULTRASOUND ; PLATEAU ; HEALTH ; OIL
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000340489100001
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/4273
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 2.Qinghai Univ, Acad Agr & Forestry, Xining, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Hu, Na,Zhang, Shijuan,Ji, Zhongyin,et al. DETERMINATION OF FATTY ACIDS IN THREE NITRARIA SPECIES BY PRECOLUMN FLUORESCENCE LABELING FOR HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION-MASS SPECTROMETRY[J]. ANALYTICAL LETTERS,2014,47(15):2475-2487.
APA	Hu, Na,Zhang, Shijuan,Ji, Zhongyin,You, Jinmao,&Suo, Yourui.(2014).DETERMINATION OF FATTY ACIDS IN THREE NITRARIA SPECIES BY PRECOLUMN FLUORESCENCE LABELING FOR HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION-MASS SPECTROMETRY.ANALYTICAL LETTERS,47(15),2475-2487.
MLA	Hu, Na,et al."DETERMINATION OF FATTY ACIDS IN THREE NITRARIA SPECIES BY PRECOLUMN FLUORESCENCE LABELING FOR HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION-MASS SPECTROMETRY".ANALYTICAL LETTERS 47.15(2014):2475-2487.