NWIPB OpenIR
Separation and Purification of Four Flavan-3-ols From Iris Lactea Pall. var. Chinensis (Fisch.) Koidz by High-Speed Counter-Current Chromatography with Flow-Rate Gradient
Lv, Huanhuan ; Ouyang, Jian ; Wang, Xiaoyan ; Ma, Xiaofeng ; Suo, Yourui ; Wang, Honglun
2015-09-14
发表期刊JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
摘要In this study, we established a flow-rate gradient separation method to isolate four flavan-3-ol compounds with high purity from the seed coat extracts of Iris lactea by high-speed counter-current chromatography (HSCCC). First, the ethyl acetate extract was pre-separated by silica column chromatography fractionation to obtain the enriched flavan-ols sample (EFS). Then, the EFS was separated by HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (0.75:12.5:1:12.5, v/v/v/v) by increasing the flow rate of the mobile phase from 1.5 to 3.0mL/min after 120min. The separation temperature was 30 degrees C, the detection wavelength was 280nm and the revolution speed was 900rpm. By the optimized HSCCC method, 300mg of the EFS was separated, yielding 58mg of procyanidin B3, 72mg of procyanidin B1, 52mg of catechin, and 18mg of procyanidin B7, at a purity of 98.23%, 99.05%, 99.03%, and 96.34% in 240min. In this work, the flavan-3-ols in I. lactea are partly reviewed and the results can provide evidence to look for other rich source of procyanidins.; In this study, we established a flow-rate gradient separation method to isolate four flavan-3-ol compounds with high purity from the seed coat extracts of Iris lactea by high-speed counter-current chromatography (HSCCC). First, the ethyl acetate extract was pre-separated by silica column chromatography fractionation to obtain the enriched flavan-ols sample (EFS). Then, the EFS was separated by HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (0.75:12.5:1:12.5, v/v/v/v) by increasing the flow rate of the mobile phase from 1.5 to 3.0mL/min after 120min. The separation temperature was 30 degrees C, the detection wavelength was 280nm and the revolution speed was 900rpm. By the optimized HSCCC method, 300mg of the EFS was separated, yielding 58mg of procyanidin B3, 72mg of procyanidin B1, 52mg of catechin, and 18mg of procyanidin B7, at a purity of 98.23%, 99.05%, 99.03%, and 96.34% in 240min. In this work, the flavan-3-ols in I. lactea are partly reviewed and the results can provide evidence to look for other rich source of procyanidins.
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/42771
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Lv, Huanhuan,Ouyang, Jian,Wang, Xiaoyan,et al. Separation and Purification of Four Flavan-3-ols From Iris Lactea Pall. var. Chinensis (Fisch.) Koidz by High-Speed Counter-Current Chromatography with Flow-Rate Gradient[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2015.
APA Lv, Huanhuan,Ouyang, Jian,Wang, Xiaoyan,Ma, Xiaofeng,Suo, Yourui,&Wang, Honglun.(2015).Separation and Purification of Four Flavan-3-ols From Iris Lactea Pall. var. Chinensis (Fisch.) Koidz by High-Speed Counter-Current Chromatography with Flow-Rate Gradient.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES.
MLA Lv, Huanhuan,et al."Separation and Purification of Four Flavan-3-ols From Iris Lactea Pall. var. Chinensis (Fisch.) Koidz by High-Speed Counter-Current Chromatography with Flow-Rate Gradient".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES (2015).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lv, Huanhuan]的文章
[Ouyang, Jian]的文章
[Wang, Xiaoyan]的文章
百度学术
百度学术中相似的文章
[Lv, Huanhuan]的文章
[Ouyang, Jian]的文章
[Wang, Xiaoyan]的文章
必应学术
必应学术中相似的文章
[Lv, Huanhuan]的文章
[Ouyang, Jian]的文章
[Wang, Xiaoyan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。