Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| A Medicago truncatula H+-pyrophosphatase gene, MtVP1, improves sucrose accumulation and anthocyanin biosynthesis in potato (Solanum tuberosum L.) | |
| Wang, J. W.1,3; Wang, H. Q.2; Xiang, W. W.3; Chai, T. Y.1 | |
| 2014 | |
| 发表期刊 | GENETICS AND MOLECULAR RESEARCH
 |
| ISSN | 1676-5680 |
| 卷号 | 13期号:2页码:3615-3626 |
| 文章类型 | Article |
| 摘要 | We recently cloned MtVP1, a type I vacuolar-type H+-translocating inorganic pyrophosphatase from Medicago truncatula. In the present study, we investigated the cellular location and the function of this H+-PPase in Arabidopsis and potato (Solanum tuberosum L.). An MtVP1::enhanced green fluorescent protein fusion was constructed, which localized to the plasma membrane of onion epidermal cells. Transgenic Arabidopsis thaliana overexpressing MtVP1 had more robust root systems and redder shoots than wild-type (WT) plants under conditions of cold stress. Furthermore, overexpression of MtVP1 in potato accelerated the formation and growth of vegetative organs. The tuber buds and stem base of transgenic potatoes became redder than those of WT plants, but flowering was delayed by approximately half a month. Interestingly, anthocyanin biosynthesis was promoted in transgenic Arabidopsis seedlings and potato tuber buds. The sucrose concentration of transgenic potato tubers and tuber buds was enhanced compared with that of WT plants. Furthermore, sucrose concentration in tubers was higher than that in tuber buds. Although there was no direct evidence to support Fuglsang's hypothetical model regarding the effects of H+-PPase on sucrose phloem loading, we speculated that sucrose concentration was increased in tuber buds owing to the increased concentration in tubers. Therefore, overexpressed MtVP1 enhanced sucrose accumulation of source organs, which might enhance sucrose transport to sink organs, thus affecting anthocyanin biosynthesis.; We recently cloned MtVP1, a type I vacuolar-type H+-translocating inorganic pyrophosphatase from Medicago truncatula. In the present study, we investigated the cellular location and the function of this H+-PPase in Arabidopsis and potato (Solanum tuberosum L.). An MtVP1::enhanced green fluorescent protein fusion was constructed, which localized to the plasma membrane of onion epidermal cells. Transgenic Arabidopsis thaliana overexpressing MtVP1 had more robust root systems and redder shoots than wild-type (WT) plants under conditions of cold stress. Furthermore, overexpression of MtVP1 in potato accelerated the formation and growth of vegetative organs. The tuber buds and stem base of transgenic potatoes became redder than those of WT plants, but flowering was delayed by approximately half a month. Interestingly, anthocyanin biosynthesis was promoted in transgenic Arabidopsis seedlings and potato tuber buds. The sucrose concentration of transgenic potato tubers and tuber buds was enhanced compared with that of WT plants. Furthermore, sucrose concentration in tubers was higher than that in tuber buds. Although there was no direct evidence to support Fuglsang's hypothetical model regarding the effects of H+-PPase on sucrose phloem loading, we speculated that sucrose concentration was increased in tuber buds owing to the increased concentration in tubers. Therefore, overexpressed MtVP1 enhanced sucrose accumulation of source organs, which might enhance sucrose transport to sink organs, thus affecting anthocyanin biosynthesis. |
| 关键词 | h+-pyrophosphatase Mtvp1 Anthocyanin Sucrose |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 关键词[WOS] | TRANSLOCATING INORGANIC PYROPHOSPHATASE ; PLASMA-MEMBRANE ; ARABIDOPSIS-THALIANA ; MEDIATED TRANSFORMATION ; PPASE GENE ; EXPRESSION ; LOCALIZATION ; GROWTH ; OVEREXPRESSION ; INDUCTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS类目 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS记录号 | WOS:000336729500132 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/4278 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Qinghai, Peoples R China 3.Yulin Univ, Coll Life Sci, Yulin, Shaanxi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, J. W.,Wang, H. Q.,Xiang, W. W.,et al. A Medicago truncatula H+-pyrophosphatase gene, MtVP1, improves sucrose accumulation and anthocyanin biosynthesis in potato (Solanum tuberosum L.)[J]. GENETICS AND MOLECULAR RESEARCH,2014,13(2):3615-3626. |
| APA | Wang, J. W.,Wang, H. Q.,Xiang, W. W.,&Chai, T. Y..(2014).A Medicago truncatula H+-pyrophosphatase gene, MtVP1, improves sucrose accumulation and anthocyanin biosynthesis in potato (Solanum tuberosum L.).GENETICS AND MOLECULAR RESEARCH,13(2),3615-3626. |
| MLA | Wang, J. W.,et al."A Medicago truncatula H+-pyrophosphatase gene, MtVP1, improves sucrose accumulation and anthocyanin biosynthesis in potato (Solanum tuberosum L.)".GENETICS AND MOLECULAR RESEARCH 13.2(2014):3615-3626. |
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