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2-(2-(Pyren-1-yl)-1H-benzo[d]imidazol-1-yl)-ethyl-4-methyl benzenesulfonate (PBITS) and its application for determination of bile acids by HPLC-FLD-MS
Xia, Lian1; Wu, Chuanxiang1; Sun, Zhiwei1; You, Jinmao1,2
2014
发表期刊ANALYTICAL METHODS
ISSN1759-9660
卷号6期号:4页码:1135-1141
文章类型Article
摘要A method of high performance liquid chromatography-fluorescence detection-mass spectrometry identification (HPLC-FLD-MS) coupled with pre-column derivatization to determine bile acids (BAs) from bio-sample has been developed here. The labeling reagent of 2-(2-(pyren-1-yl)-1H-benzo[d] imidazol-1-yl)-ethyl-4-methyl benzenesulfonate (PBITS) contains six benzene rings and two weak basic nitrogens in the structure, resulting in a higher degree of conjugation and perfect ionization efficiency. The PBITS could easily and quickly label BAs at the optimized derivatization conditions and the labeling yield was close to 100%. Separation of the derivatized BAs exhibited a good baseline resolution in combination with a gradient elution on a reversed-phase Hypersil BDS C-18 column. Calculated detection limits for HPLC-FLD, at a signal-to-noise ratio of 3, were 10.09-16.33 fmol. Excellent linear responses were observed with correlation coefficients >0.9995. The mean inter-day precision for all standards was <3.63% and the experimental recoveries were 89.9-106.3%. Good compositional data were obtained from the analysis of the extracted BAs from pig bile sample. Therefore, the facile PBITS derivatization coupled with HPLC-FLD-MS analysis allowed the development of a highly sensitive method for the quantization of trace levels of BAs from biological samples.; A method of high performance liquid chromatography-fluorescence detection-mass spectrometry identification (HPLC-FLD-MS) coupled with pre-column derivatization to determine bile acids (BAs) from bio-sample has been developed here. The labeling reagent of 2-(2-(pyren-1-yl)-1H-benzo[d] imidazol-1-yl)-ethyl-4-methyl benzenesulfonate (PBITS) contains six benzene rings and two weak basic nitrogens in the structure, resulting in a higher degree of conjugation and perfect ionization efficiency. The PBITS could easily and quickly label BAs at the optimized derivatization conditions and the labeling yield was close to 100%. Separation of the derivatized BAs exhibited a good baseline resolution in combination with a gradient elution on a reversed-phase Hypersil BDS C-18 column. Calculated detection limits for HPLC-FLD, at a signal-to-noise ratio of 3, were 10.09-16.33 fmol. Excellent linear responses were observed with correlation coefficients >0.9995. The mean inter-day precision for all standards was <3.63% and the experimental recoveries were 89.9-106.3%. Good compositional data were obtained from the analysis of the extracted BAs from pig bile sample. Therefore, the facile PBITS derivatization coupled with HPLC-FLD-MS analysis allowed the development of a highly sensitive method for the quantization of trace levels of BAs from biological samples.
WOS标题词Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine ; Technology
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; LASER-INDUCED FLUORESCENCE ; GAS-CHROMATOGRAPHY ; HUMAN-SERUM ; INTRAHEPATIC CHOLESTASIS ; CARBOXYLIC-ACIDS ; PROFILES ; REAGENT ; TRIFLUOROMETHANESULFONATE
收录类别SCI
语种英语
WOS研究方向Chemistry ; Food Science & Technology ; Spectroscopy
WOS类目Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
WOS记录号WOS:000330795200024
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/4284
专题中国科学院西北高原生物研究所
作者单位1.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China
2.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining 810001, Peoples R China
推荐引用方式
GB/T 7714
Xia, Lian,Wu, Chuanxiang,Sun, Zhiwei,et al. 2-(2-(Pyren-1-yl)-1H-benzo[d]imidazol-1-yl)-ethyl-4-methyl benzenesulfonate (PBITS) and its application for determination of bile acids by HPLC-FLD-MS[J]. ANALYTICAL METHODS,2014,6(4):1135-1141.
APA Xia, Lian,Wu, Chuanxiang,Sun, Zhiwei,&You, Jinmao.(2014).2-(2-(Pyren-1-yl)-1H-benzo[d]imidazol-1-yl)-ethyl-4-methyl benzenesulfonate (PBITS) and its application for determination of bile acids by HPLC-FLD-MS.ANALYTICAL METHODS,6(4),1135-1141.
MLA Xia, Lian,et al."2-(2-(Pyren-1-yl)-1H-benzo[d]imidazol-1-yl)-ethyl-4-methyl benzenesulfonate (PBITS) and its application for determination of bile acids by HPLC-FLD-MS".ANALYTICAL METHODS 6.4(2014):1135-1141.
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