An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography

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	An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography
	Zou, Denglang ; Chen, Tao ; Chen, Chen ; Li, Hongmei ; Liu, Yongling ; Li, Yulin
	2016-08-01
发表期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE
摘要	In this article, macroporous resin column chromatography and preparative high-performance liquid chromatography were applied for preparation of gallic acid from Terminalia bellirica (Gaertn.) Roxb. In the first step, six kinds of resins were investigated by adsorption and desorption tests and AB-8 macroporous resin was selected for the enrichment of gallic acid. As a result, 20 g of gallic acid at a purity of 71% could be separated from 100 g of crude extract in which the content of gallic acid was 16.7% and the recovery of gallic acid reached 85.0%. In the second step, preparative high-performance liquid chromatography was selected to purify gallic acid. As a result, 640 mg of gallic acid at a purity of 99.1% was obtained from 1 g of sample in 35 min. The results demonstrated that macroporous resin coupled with preparative high-performance liquid chromatography was suitable for preparation of gallic acid from T. bellirica (Gaertn.) Roxb.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/43256
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zou, Denglang,Chen, Tao,Chen, Chen,et al. An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2016.
APA	Zou, Denglang,Chen, Tao,Chen, Chen,Li, Hongmei,Liu, Yongling,&Li, Yulin.(2016).An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography.JOURNAL OF CHROMATOGRAPHIC SCIENCE.
MLA	Zou, Denglang,et al."An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography".JOURNAL OF CHROMATOGRAPHIC SCIENCE (2016).