Hypaphorine, an Indole Alkaloid Isolated from Caragana korshinskii Kom., Inhibites 3T3-L1 Adipocyte Differentiation and Improves Insulin Sensitivity in Vitro

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	Hypaphorine, an Indole Alkaloid Isolated from Caragana korshinskii Kom., Inhibites 3T3-L1 Adipocyte Differentiation and Improves Insulin Sensitivity in Vitro
	Luan, Guangxiang ; Tie, Fangfang ; Yuan, Zhenzhen ; Li, Gang ; He, Jie ; Wang, Zhenhua ; Wang, Honglun
	2017-07-01
发表期刊	CHEMISTRY & BIODIVERSITY
摘要	Obesity, a major health problem worldwide, is a complex multifactorial chronic disease that increases the risk for insulin resistance, type 2 diabetes, coronary heart disease, and hypertension. In this study, we assessed methods to isolate hypaphorine, a potent drug candidate for obesity and insulin resistance. Semi-preparative reversed-phase liquid chromatography (semi-preparative RPLC) was established as a method to separate three compounds, adenosine, l-tryptophan, and hypaphorine, from the crude extracts of Caragana korshinskii Kom. Due to its specific chemical structure, the effect of hypaphorine on differentiation and dexamethasone (DXM) induced insulin resistance of 3T3-L1 cells was investigated. The structures of the three compounds were confirmed by UV, H-1-NMR, and C-13-NMR analysis and compared with published data. The activity results indicated that hypaphorine prevented the differentiation of 3T3-L1 preadipocytes into adipocytes by down-regulating hormone-stimulated protein expression of peroxisome proliferator activated receptor (PPAR) and CCAAT/enhancer binding protein (C/EBP), and their downstream targets, sterol regulatory element binding protein 1 c (SREBP1c) and fatty acid synthase (FAS). Hypaphorine also alleviated DXM-induced insulin resistance in differentiated 3T3-L1 adipocytes via increasing the phosphorylation level of Akt2, a key protein in the insulin signaling pathway. Taken together, we suggest that the method can be applied to large-scale extraction and large-quantity preparation of hypaphorine for treatment of obesity and insulin resistance.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/43528
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Luan, Guangxiang,Tie, Fangfang,Yuan, Zhenzhen,et al. Hypaphorine, an Indole Alkaloid Isolated from Caragana korshinskii Kom., Inhibites 3T3-L1 Adipocyte Differentiation and Improves Insulin Sensitivity in Vitro[J]. CHEMISTRY & BIODIVERSITY,2017.
APA	Luan, Guangxiang.,Tie, Fangfang.,Yuan, Zhenzhen.,Li, Gang.,He, Jie.,...&Wang, Honglun.(2017).Hypaphorine, an Indole Alkaloid Isolated from Caragana korshinskii Kom., Inhibites 3T3-L1 Adipocyte Differentiation and Improves Insulin Sensitivity in Vitro.CHEMISTRY & BIODIVERSITY.
MLA	Luan, Guangxiang,et al."Hypaphorine, an Indole Alkaloid Isolated from Caragana korshinskii Kom., Inhibites 3T3-L1 Adipocyte Differentiation and Improves Insulin Sensitivity in Vitro".CHEMISTRY & BIODIVERSITY (2017).