Separation of six xanthones from Swertia franchetiana by high-speed countercurrent chromatography

NWIPB OpenIR

	Separation of six xanthones from Swertia franchetiana by high-speed countercurrent chromatography
	Li, Hongmei ; Chen, Tao ; Sun, Jing ; Wang, Weiqing ; Li, Yulin
	2017-06-01
发表期刊	JOURNAL OF SEPARATION SCIENCE
摘要	In our present study, two groups of xanthones isomers (1-hydroxy-3,5,8-trimethoxyxanthone and 1-hydroxy-3,7,8-trimethoxyxanthone; 1,8-dihydroxy-3,7-dimethoxyxanthone and 1,8-dihydroxy-3,5-dimethanolxanthone) and other two xanthones (3-methoxy-1,5,8-trihydroxyxanthone and 3,5-dimethoxy-1-hydroxyxanthone) were separated from Swertia franchetiana. First, a solvent system composed of petroleum ether/methanol/water (2:1:0.6, v/v) was developed for the liquid-liquid extraction of these xanthones from the crude extract. Then, an efficient method was established for the one-step separation of these six xanthones by high-speed countercurrent chromatography using n-hexane/ethyl acetate/methanol/ethanol/water (HEMEW; 6:4:4:2:4, v/v) as the solvent system. The results showed that liquid-liquid extraction could be well developed for efficient enrichment of target compounds. Additionally, high-speed countercurrent chromatography could be a powerful technology for separation xanthones isomers. It was found ethanol could be a good methanol substitute when the HEMEW system could not provide good separation factors.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/43539
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, Hongmei,Chen, Tao,Sun, Jing,et al. Separation of six xanthones from Swertia franchetiana by high-speed countercurrent chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2017.
APA	Li, Hongmei,Chen, Tao,Sun, Jing,Wang, Weiqing,&Li, Yulin.(2017).Separation of six xanthones from Swertia franchetiana by high-speed countercurrent chromatography.JOURNAL OF SEPARATION SCIENCE.
MLA	Li, Hongmei,et al."Separation of six xanthones from Swertia franchetiana by high-speed countercurrent chromatography".JOURNAL OF SEPARATION SCIENCE (2017).