CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides

NWIPB OpenIR

	CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides
	Ding, Chenxu ; Sun, Xuejun ; Zhao, Xianen ; Zhao, Wenchen ; Li, Yulin ; Wang, Honglun ; Suo, Yourui ; You, Jinmao
	2008-02-01
发表期刊	CHROMATOGRAPHIA ; Ding Chenxu,Sun Xuejun,Zhao Xianen,Zhao Wenchen,Li Yulin, Wang Honglun,Suo Yourui,You Jinmao.CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides.CHROMATOGRAPHIA,2008,67(3-4):245-251
摘要	A pre-column derivatization method for sensitive determination of oligopeptides, using the tagging reagent 2-(9-carbazole)ethyl chloroformate (CEOC-Cl) followed by capillary electrophoresis (CE) with diode-array detection, has been developed. Maximum yield close to 100% were observed when a three to fourfold molar excess of reagent was used at pH 9.0-10.0. Excess reagent was extracted with n-hexane-ethyl acetate 9:1-10:1 (v/v); this enabled direct analysis using CE with no significant disturbance from the major fluorescent reagent degradation by-products. The effects on the results of buffer pH and of SDS and organic modifier concentrations were examined. Good baseline resolution in the separation of five CEOC-peptides was achieved with a 48.5-cm total length (effective length 40 cm) 50-mu m inner diameter capillary column.; A pre-column derivatization method for sensitive determination of oligopeptides, using the tagging reagent 2-(9-carbazole)ethyl chloroformate (CEOC-Cl) followed by capillary electrophoresis (CE) with diode-array detection, has been developed. Maximum yield close to 100% were observed when a three to fourfold molar excess of reagent was used at pH 9.0-10.0. Excess reagent was extracted with n-hexane-ethyl acetate 9:1-10:1 (v/v); this enabled direct analysis using CE with no significant disturbance from the major fluorescent reagent degradation by-products. The effects on the results of buffer pH and of SDS and organic modifier concentrations were examined. Good baseline resolution in the separation of five CEOC-peptides was achieved with a 48.5-cm total length (effective length 40 cm) 50-mu m inner diameter capillary column.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/45634
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Ding, Chenxu,Sun, Xuejun,Zhao, Xianen,et al. CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides[J]. CHROMATOGRAPHIA, Ding Chenxu,Sun Xuejun,Zhao Xianen,Zhao Wenchen,Li Yulin, Wang Honglun,Suo Yourui,You Jinmao.CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides.CHROMATOGRAPHIA,2008,67(3-4):245-251,2008.
APA	Ding, Chenxu.,Sun, Xuejun.,Zhao, Xianen.,Zhao, Wenchen.,Li, Yulin.,...&You, Jinmao.(2008).CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides.CHROMATOGRAPHIA.
MLA	Ding, Chenxu,et al."CE determination of 2-(9-carbazole)ethyl chloroformate-labeled oligopeptides".CHROMATOGRAPHIA (2008).