Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii

NWIPB OpenIR

	Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii
	Xue, Chun-Ying ; Li, De-Zhu ; Lu, Jin-Mei ; Yang, Jun-Bo ; Liu, Jian-Quan
	2006-10-01
发表期刊	PLANTA MEDICA ; Xue ChunYing, Li DeZhu,Lu JinMei, Yang JunBo, Liu JianQuan. Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii. PLANTA MEDICA,2006,72(13):1223-1226
摘要	Swertia mussotii is an important species in Tibetan folk medicine. However, it is quite expensive and frequently adulterated, so reliable methods for authentication of putative specimens and preparations of the species are needed to protect consumers and to support conservation measures. We show here that the chloroplast (cp) DNA rpl16 intron has limited utility for differentiating S. mussotii from closely related species, since the cpDNA rpl16 sequences are identical in S. mussotii and two other species of Swertia. However, the rDNA internal transcribed spacer (ITS) sequences differ significantly between S. mussotii and all of 13 tested potential adulterants. Thus, the ITS region provides a robust molecular marker for differentiating the medicinal S. mussotii from related adulterants. Therefore, a pair of allele-specific diagnostic primers based on the divergent ITS region was designed to distinguish S. mussotii from the other species. Authentication by allele-specific diagnostic PCR using these primers is convenient, effective and both simpler and less time-consuming than sequencing the ITS region.; Swertia mussotii is an important species in Tibetan folk medicine. However, it is quite expensive and frequently adulterated, so reliable methods for authentication of putative specimens and preparations of the species are needed to protect consumers and to support conservation measures. We show here that the chloroplast (cp) DNA rpl16 intron has limited utility for differentiating S. mussotii from closely related species, since the cpDNA rpl16 sequences are identical in S. mussotii and two other species of Swertia. However, the rDNA internal transcribed spacer (ITS) sequences differ significantly between S. mussotii and all of 13 tested potential adulterants. Thus, the ITS region provides a robust molecular marker for differentiating the medicinal S. mussotii from related adulterants. Therefore, a pair of allele-specific diagnostic primers based on the divergent ITS region was designed to distinguish S. mussotii from the other species. Authentication by allele-specific diagnostic PCR using these primers is convenient, effective and both simpler and less time-consuming than sequencing the ITS region.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/45703
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Xue, Chun-Ying,Li, De-Zhu,Lu, Jin-Mei,et al. Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii[J]. PLANTA MEDICA, Xue ChunYing, Li DeZhu,Lu JinMei, Yang JunBo, Liu JianQuan. Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii. PLANTA MEDICA,2006,72(13):1223-1226,2006.
APA	Xue, Chun-Ying,Li, De-Zhu,Lu, Jin-Mei,Yang, Jun-Bo,&Liu, Jian-Quan.(2006).Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii.PLANTA MEDICA.
MLA	Xue, Chun-Ying,et al."Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii".PLANTA MEDICA (2006).