Synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process

NWIPB OpenIR

	Synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process
	Zhang, Lianquan ; Zhang, Li ; Luo, Jiangtao ; Chen, Wenjie ; Hao, Ming ; Liu, Baolong ; Yan, Zehong ; Zhang, Bo ; Zhang, Huaigang ; Zheng, Youliang ; Liu, Dengcai ; Yen, Yang
	2011-02-01
发表期刊	JOURNAL OF GENETICS AND GENOMICS ; Zhang, LQ; Zhang, L; Luo, JT; Chen, WJ; Hao, M; Liu, BL; Yan, ZH; Zhang, B; Zhang, HG; Zheng, YL; Liu, DC; Yen, Y.Synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process,JOURNAL OF GENETICS AND GENOMICS,2011,38(2):89-94
摘要	Doubled haploid (DH) populations are useful to scientists and breeders in both crop improvement and basic research. Current methods of producing DHs usually need in vitro culture for extracting haploids and chemical treatment for chromosome doubling. This report describes a simple method for synthesizing DHs (SynDH) especially for allopolyploid species by utilizing meiotic restitution genes. The method involves three steps: hybridization to induce recombination, interspecific hybridization to extract haploids, and spontaneous chromosome doubling by selfing the interspecific F(1)s. DHs produced in this way contain recombinant chromosomes in the genome(s) of interest in a homogeneous background. No special equipment or treatments are involved in the DH production and it can be easily applied in any breeding and/or genetic program. Triticum turgidum L. and Aegilops tauschii Coss, the two ancestral species of common wheat (Triticum aestivum L.) and molecular markers were used to demonstrate the SynDH method.; Doubled haploid (DH) populations are useful to scientists and breeders in both crop improvement and basic research. Current methods of producing DHs usually need in vitro culture for extracting haploids and chemical treatment for chromosome doubling. This report describes a simple method for synthesizing DHs (SynDH) especially for allopolyploid species by utilizing meiotic restitution genes. The method involves three steps: hybridization to induce recombination, interspecific hybridization to extract haploids, and spontaneous chromosome doubling by selfing the interspecific F(1)s. DHs produced in this way contain recombinant chromosomes in the genome(s) of interest in a homogeneous background. No special equipment or treatments are involved in the DH production and it can be easily applied in any breeding and/or genetic program. Triticum turgidum L. and Aegilops tauschii Coss, the two ancestral species of common wheat (Triticum aestivum L.) and molecular markers were used to demonstrate the SynDH method.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/45934
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhang, Lianquan,Zhang, Li,Luo, Jiangtao,et al. Synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process[J]. JOURNAL OF GENETICS AND GENOMICS, Zhang, LQ; Zhang, L; Luo, JT; Chen, WJ; Hao, M; Liu, BL; Yan, ZH; Zhang, B; Zhang, HG; Zheng, YL; Liu, DC; Yen, Y.Synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process,JOURNAL OF GENETICS AND GENOMICS,2011,38(2):89-94,2011.
APA	Zhang, Lianquan.,Zhang, Li.,Luo, Jiangtao.,Chen, Wenjie.,Hao, Ming.,...&Yen, Yang.(2011).Synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process.JOURNAL OF GENETICS AND GENOMICS.
MLA	Zhang, Lianquan,et al."Synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process".JOURNAL OF GENETICS AND GENOMICS (2011).