Extraction of Microula sikkimensis seed oil and simultaneous analysis of saturated and unsaturated fatty acids by fluorescence detection with reversed-phase HPLC

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	Extraction of Microula sikkimensis seed oil and simultaneous analysis of saturated and unsaturated fatty acids by fluorescence detection with reversed-phase HPLC
	Cao, Yue ; Suo, Yourui
	2010-02-01
发表期刊	JOURNAL OF FOOD COMPOSITION AND ANALYSIS ; Cao, Y; Suo, YR.Extraction of Microula sikkimensis seed oil and simultaneous analysis of saturated and unsaturated fatty acids by fluorescence detection with reversed-phase HPLC,JOURNAL OF FOOD COMPOSITION AND ANALYSIS,2010,23(1):100-106
摘要	The seed oil of Microula sikkimensis Hemsl. was extracted by supercritical carbon dioxide extraction, microwave-assisted reflux extraction, ultrasound-assisted extraction and solvent reflux extraction. The experimental parameters of supercritical carbon dioxide extraction which is classic in these extraction techniques including pressure, temperature, particle size and extraction time were optimized. A simple and sensitive method for the simultaneous determination of 39 kinds of saturated and unsaturated fatty acids in seed oil using high performance liquid chromatography (HPLC) with fluorescence detection following pre-column derivatization with 1-[2-(p-toluenesulfonate)-ethyl]-2-phenylimidazole-[4,5-f]-9,10-phenanthrene (TSPP) has been developed. Fatty acid derivatives were separated on a reversed-phase Eclipse XDB-C(8) column in conjunction with a gradient elution. Quantitative linear range of 39 fatty acids was 0.014-14.29 mu mol/L, and excellent linear responses were observed with correlation coefficients more than 0.9992. Detection limits were in the range of 3.24-36.97 fmol (10 mu L, S/N 3: I). Stability of derivatives, method repeatability and recovery were evaluated and the results were excellent for efficient HPLC analysis. Fatty acids in M. sikkimensis Hemsl. seed oil with or without saponification by different extraction methods were analyzed and compared. The facile TSPP derivatization coupled with HPLC fluorescence detection allowed for the quantitation of short- and long-chain fatty acids from biological and natural environmental samples. (C) 2009 Elsevier Inc. All rights reserved.; The seed oil of Microula sikkimensis Hemsl. was extracted by supercritical carbon dioxide extraction, microwave-assisted reflux extraction, ultrasound-assisted extraction and solvent reflux extraction. The experimental parameters of supercritical carbon dioxide extraction which is classic in these extraction techniques including pressure, temperature, particle size and extraction time were optimized. A simple and sensitive method for the simultaneous determination of 39 kinds of saturated and unsaturated fatty acids in seed oil using high performance liquid chromatography (HPLC) with fluorescence detection following pre-column derivatization with 1-[2-(p-toluenesulfonate)-ethyl]-2-phenylimidazole-[4,5-f]-9,10-phenanthrene (TSPP) has been developed. Fatty acid derivatives were separated on a reversed-phase Eclipse XDB-C(8) column in conjunction with a gradient elution. Quantitative linear range of 39 fatty acids was 0.014-14.29 mu mol/L, and excellent linear responses were observed with correlation coefficients more than 0.9992. Detection limits were in the range of 3.24-36.97 fmol (10 mu L, S/N 3: I). Stability of derivatives, method repeatability and recovery were evaluated and the results were excellent for efficient HPLC analysis. Fatty acids in M. sikkimensis Hemsl. seed oil with or without saponification by different extraction methods were analyzed and compared. The facile TSPP derivatization coupled with HPLC fluorescence detection allowed for the quantitation of short- and long-chain fatty acids from biological and natural environmental samples. (C) 2009 Elsevier Inc. All rights reserved.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/46015
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Cao, Yue,Suo, Yourui. Extraction of Microula sikkimensis seed oil and simultaneous analysis of saturated and unsaturated fatty acids by fluorescence detection with reversed-phase HPLC[J]. JOURNAL OF FOOD COMPOSITION AND ANALYSIS, Cao, Y; Suo, YR.Extraction of Microula sikkimensis seed oil and simultaneous analysis of saturated and unsaturated fatty acids by fluorescence detection with reversed-phase HPLC,JOURNAL OF FOOD COMPOSITION AND ANALYSIS,2010,23(1):100-106,2010.
APA	Cao, Yue,&Suo, Yourui.(2010).Extraction of Microula sikkimensis seed oil and simultaneous analysis of saturated and unsaturated fatty acids by fluorescence detection with reversed-phase HPLC.JOURNAL OF FOOD COMPOSITION AND ANALYSIS.
MLA	Cao, Yue,et al."Extraction of Microula sikkimensis seed oil and simultaneous analysis of saturated and unsaturated fatty acids by fluorescence detection with reversed-phase HPLC".JOURNAL OF FOOD COMPOSITION AND ANALYSIS (2010).