A Novel Labeling Reagent of 2-(12-Benzo[b]acridin-5-(12H)-yl)-acetohydrazide for Determination of Saturated and Unsaturated Fatty Acids in Traditional Chinese Herbs by HPLC-APCI-MS

NWIPB OpenIR

	A Novel Labeling Reagent of 2-(12-Benzo[b]acridin-5-(12H)-yl)-acetohydrazide for Determination of Saturated and Unsaturated Fatty Acids in Traditional Chinese Herbs by HPLC-APCI-MS
	Xie, Yinfeng ; Li, Guoliang ; You, Jinmao ; Bai, Xinwei ; Wang, Chengyan ; Zhang, Lin ; Zhao, Fuhua ; Wu, Xiaoyun ; Ji, Zhongyin ; Sun, Zhiwei
	2012-06-01
发表期刊	CHROMATOGRAPHIA ; Li, C; Sang-lao; Leng-bencairang; Xia, ZJ; Du, YZ; Wei, LX.A Novel Labeling Reagent of 2-(12-Benzo[b]acridin-5-(12H)-yl)-acetohydrazide for Determination of Saturated and Unsaturated Fatty Acids in Traditional Chinese Herbs by HPLC-APCI-MS,CHROMATOGRAPHIA,2012,75(11-12):571-583
摘要	A new fluorescence labeling reagent 2-(12-benzo[b]acridin-5(12H)-yl)-acetohydrazide (BAAH) has been designed for fatty acids labeling. Eleven fatty acids containing seven saturated and four unsaturated fatty acids were used to evaluate the analytical potential of this reagent. The labeling reaction of BAAH with fatty acids was completed at 85 A degrees C for 60 min using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC center dot HCl) as the condensing agent. Separation of the derivatized fatty acids was carried out on a reversed-phase Thermo Hypersil Gold C18 column (4.6 mm x 250 mm, 5 mu m) in combination with a gradient elution with a good baseline resolution. The fluorescence excitation and emission wavelengths were set at lambda(ex) 280 and lambda(em) 510 nm, respectively. The identification was carried out by the online APCI-MS in positive-ion detection mode. Linear correlation coefficients for all fatty acid derivatives were of > 0.9994. Detection limits, at a signal-to-noise ratio of 3:1, were 3.89-12.5 nmol L-1 for the labeled fatty acids. The developed method was successfully applied to the accurate determination of fatty acids in five traditional Chinese herbs with satisfactory results.; A new fluorescence labeling reagent 2-(12-benzo[b]acridin-5(12H)-yl)-acetohydrazide (BAAH) has been designed for fatty acids labeling. Eleven fatty acids containing seven saturated and four unsaturated fatty acids were used to evaluate the analytical potential of this reagent. The labeling reaction of BAAH with fatty acids was completed at 85 A degrees C for 60 min using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC center dot HCl) as the condensing agent. Separation of the derivatized fatty acids was carried out on a reversed-phase Thermo Hypersil Gold C18 column (4.6 mm x 250 mm, 5 mu m) in combination with a gradient elution with a good baseline resolution. The fluorescence excitation and emission wavelengths were set at lambda(ex) 280 and lambda(em) 510 nm, respectively. The identification was carried out by the online APCI-MS in positive-ion detection mode. Linear correlation coefficients for all fatty acid derivatives were of > 0.9994. Detection limits, at a signal-to-noise ratio of 3:1, were 3.89-12.5 nmol L-1 for the labeled fatty acids. The developed method was successfully applied to the accurate determination of fatty acids in five traditional Chinese herbs with satisfactory results.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/47053
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Xie, Yinfeng,Li, Guoliang,You, Jinmao,et al. A Novel Labeling Reagent of 2-(12-Benzo[b]acridin-5-(12H)-yl)-acetohydrazide for Determination of Saturated and Unsaturated Fatty Acids in Traditional Chinese Herbs by HPLC-APCI-MS[J]. CHROMATOGRAPHIA, Li, C; Sang-lao; Leng-bencairang; Xia, ZJ; Du, YZ; Wei, LX.A Novel Labeling Reagent of 2-(12-Benzo[b]acridin-5-(12H)-yl)-acetohydrazide for Determination of Saturated and Unsaturated Fatty Acids in Traditional Chinese Herbs by HPLC-APCI-MS,CHROMATOGRAPHIA,2012,75(11-12):571-583,2012.
APA	Xie, Yinfeng.,Li, Guoliang.,You, Jinmao.,Bai, Xinwei.,Wang, Chengyan.,...&Sun, Zhiwei.(2012).A Novel Labeling Reagent of 2-(12-Benzo[b]acridin-5-(12H)-yl)-acetohydrazide for Determination of Saturated and Unsaturated Fatty Acids in Traditional Chinese Herbs by HPLC-APCI-MS.CHROMATOGRAPHIA.
MLA	Xie, Yinfeng,et al."A Novel Labeling Reagent of 2-(12-Benzo[b]acridin-5-(12H)-yl)-acetohydrazide for Determination of Saturated and Unsaturated Fatty Acids in Traditional Chinese Herbs by HPLC-APCI-MS".CHROMATOGRAPHIA (2012).