A sensitive and selective HPLC-FLD method with fluorescent labeling for simultaneous detection of bile acid and free fatty acid in human serum

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	A sensitive and selective HPLC-FLD method with fluorescent labeling for simultaneous detection of bile acid and free fatty acid in human serum
	Li, Guo-Liang ; Chen, Guang ; Liu, Yue-Qiu ; Jing, Nian-Hua ; You, Jin-Mao
	2012-05-01
发表期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ; Huang, SL; Yu, RT; Gong, J; Feng, Y; Dai, YL; Hu, F; Hu, YH; Tao, YD; Leng, Y.A sensitive and selective HPLC-FLD method with fluorescent labeling for simultaneous detection of bile acid and free fatty acid in human serum,JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2012,895():191-195
摘要	A sensitive and selective method using 2(7H-dibenzo[a,g]carbazol-7-yl)ethyl 4-methylbenzenesulfonate (DBCETS) as a new fluorescent labeling reagent has been proposed for simultaneously detecting BA and FFA by HPLC with fluorescence detector. The developed method offered the low detection limits of 0.42-0.70 and 0.28-0.57 ng/mL for BA and FFA, respectively. Compared with the reported methods, the proposed method here is capable of offering higher detection sensitivity and selectivity, with less cost and lower volume of sample preparation. This method was validated to ensure high accuracy and precision, and the reliability of its results. When applied to the serum samples of healthy volunteers and patients with hepatic carcinoma, it showed excellent applicability. (C) 2012 Elsevier B.V. All rights reserved.; A sensitive and selective method using 2(7H-dibenzo[a,g]carbazol-7-yl)ethyl 4-methylbenzenesulfonate (DBCETS) as a new fluorescent labeling reagent has been proposed for simultaneously detecting BA and FFA by HPLC with fluorescence detector. The developed method offered the low detection limits of 0.42-0.70 and 0.28-0.57 ng/mL for BA and FFA, respectively. Compared with the reported methods, the proposed method here is capable of offering higher detection sensitivity and selectivity, with less cost and lower volume of sample preparation. This method was validated to ensure high accuracy and precision, and the reliability of its results. When applied to the serum samples of healthy volunteers and patients with hepatic carcinoma, it showed excellent applicability. (C) 2012 Elsevier B.V. All rights reserved.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/47061
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, Guo-Liang,Chen, Guang,Liu, Yue-Qiu,et al. A sensitive and selective HPLC-FLD method with fluorescent labeling for simultaneous detection of bile acid and free fatty acid in human serum[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, Huang, SL; Yu, RT; Gong, J; Feng, Y; Dai, YL; Hu, F; Hu, YH; Tao, YD; Leng, Y.A sensitive and selective HPLC-FLD method with fluorescent labeling for simultaneous detection of bile acid and free fatty acid in human serum,JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2012,895():191-195,2012.
APA	Li, Guo-Liang,Chen, Guang,Liu, Yue-Qiu,Jing, Nian-Hua,&You, Jin-Mao.(2012).A sensitive and selective HPLC-FLD method with fluorescent labeling for simultaneous detection of bile acid and free fatty acid in human serum.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES.
MLA	Li, Guo-Liang,et al."A sensitive and selective HPLC-FLD method with fluorescent labeling for simultaneous detection of bile acid and free fatty acid in human serum".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2012).