Determination of the carbohydrates from Notopterygium forbesii Boiss by HPLC with fluorescence detection

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	Determination of the carbohydrates from Notopterygium forbesii Boiss by HPLC with fluorescence detection
	Zhang, Shijuan ; Li, Chunli ; Zhou, Guoying ; Che, Guodong ; You, Jinmao ; Suo, Yourui ; You, JM (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China.
	2013-09-12
发表期刊	CARBOHYDRATE POLYMERS ; Zhang, SJ; Li, CL; Zhou, GY; Che, GD; You, JM; Suo, YR.Determination of the carbohydrates from Notopterygium forbesii Boiss by HPLC with fluorescence detection,CARBOHYDRATE POLYMERS,2013,97(2):794-799
摘要	A sensitive pre-column derivatization method wasdeveloped for analysis of carbohydrates by HPLC with fluorescence detection. The introduction of 2-(12-benzo[b]acridin-5(12H)-yl)-acetohydrazide (BAAH) with excellent fluorescence property into the molecules of monosaccharides greatly enhanced the HPLC sensitivity of the analytes. Meanwhile, derivatization with BAAH also greatly increased the hydrophobicity of the monosaccharides and made them elute at increased retention times. The monosaccharides with similar properties therefore could be completely separated due to the increased interaction between the analytes and the column. Component monosaccharides of the polysaccharides obtained from the roots, stems and leaves of Notopterygium forbesii Boiss (NF) were analyzed by the developed method. The results indicated that the polysaccharides of NF were mainly composed of D-galactose and D-glucose. This is the first systematic study of the sugar composition of the polysaccharides of NF. It will be helpful for the quality control of NF. (C) 2013 Elsevier Ltd. All rights reserved.; A sensitive pre-column derivatization method wasdeveloped for analysis of carbohydrates by HPLC with fluorescence detection. The introduction of 2-(12-benzo[b]acridin-5(12H)-yl)-acetohydrazide (BAAH) with excellent fluorescence property into the molecules of monosaccharides greatly enhanced the HPLC sensitivity of the analytes. Meanwhile, derivatization with BAAH also greatly increased the hydrophobicity of the monosaccharides and made them elute at increased retention times. The monosaccharides with similar properties therefore could be completely separated due to the increased interaction between the analytes and the column. Component monosaccharides of the polysaccharides obtained from the roots, stems and leaves of Notopterygium forbesii Boiss (NF) were analyzed by the developed method. The results indicated that the polysaccharides of NF were mainly composed of D-galactose and D-glucose. This is the first systematic study of the sugar composition of the polysaccharides of NF. It will be helpful for the quality control of NF. (C) 2013 Elsevier Ltd. All rights reserved.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/47259
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhang, Shijuan,Li, Chunli,Zhou, Guoying,et al. Determination of the carbohydrates from Notopterygium forbesii Boiss by HPLC with fluorescence detection[J]. CARBOHYDRATE POLYMERS, Zhang, SJ; Li, CL; Zhou, GY; Che, GD; You, JM; Suo, YR.Determination of the carbohydrates from Notopterygium forbesii Boiss by HPLC with fluorescence detection,CARBOHYDRATE POLYMERS,2013,97(2):794-799,2013.
APA	Zhang, Shijuan.,Li, Chunli.,Zhou, Guoying.,Che, Guodong.,You, Jinmao.,...&You, JM .(2013).Determination of the carbohydrates from Notopterygium forbesii Boiss by HPLC with fluorescence detection.CARBOHYDRATE POLYMERS.
MLA	Zhang, Shijuan,et al."Determination of the carbohydrates from Notopterygium forbesii Boiss by HPLC with fluorescence detection".CARBOHYDRATE POLYMERS (2013).