Anticancer and immunostimulating activities of a novel homogalacturonan from Hippophae rhamnoides L. berry

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	Anticancer and immunostimulating activities of a novel homogalacturonan from Hippophae rhamnoides L. berry
	Wang, Hailiang ; Gao, Tingting ; Du, Yuzhi ; Yang, Hongxia ; Wei, Lixin ; Bi, Hongtao ; Ni, Weihua
	2015-10-20
发表期刊	CARBOHYDRATE POLYMERS
摘要	Our previous study isolated an anti-fatigue polysaccharide (HRWP) from the Hippophae rhamnoides berry. In this study, using ion-exchange chromatography and gel filtration chromatography in turn, a watersoluble homogenous polysaccharide HRWP-A was isolated from HRWP. Structural analysis determined that HRWP-A was a polysaccharide with repeating units of (1 -> 4)-beta-D-galactopyranosyluronic residues, of which 85.16% were esterified with methyl groups. An antitumor activity assay showed that HRWP-A could significantly inhibit the Lewis lung carcinoma (LLC) growth in tumor-bearing mice. Further experiments suggested that the antitumor effect of HRWP-A might be mediated through immunostimulating activity, as it enhances the lymphocyte proliferation, augments the macrophage activities, as well as promoting NK cell activity and CTL cytotoxicity in tumor-bearing mice. To our knowledge, this is the first report on a natural antitumor high-methoxyl homogalacturonan pectin from the H. rhamnoides berry-a compound that acts as a potential immunostimulant and anticancer adjuvant. (C) 2015 Elsevier Ltd. All rights reserved.; Our previous study isolated an anti-fatigue polysaccharide (HRWP) from the Hippophae rhamnoides berry. In this study, using ion-exchange chromatography and gel filtration chromatography in turn, a watersoluble homogenous polysaccharide HRWP-A was isolated from HRWP. Structural analysis determined that HRWP-A was a polysaccharide with repeating units of (1 -> 4)-beta-D-galactopyranosyluronic residues, of which 85.16% were esterified with methyl groups. An antitumor activity assay showed that HRWP-A could significantly inhibit the Lewis lung carcinoma (LLC) growth in tumor-bearing mice. Further experiments suggested that the antitumor effect of HRWP-A might be mediated through immunostimulating activity, as it enhances the lymphocyte proliferation, augments the macrophage activities, as well as promoting NK cell activity and CTL cytotoxicity in tumor-bearing mice. To our knowledge, this is the first report on a natural antitumor high-methoxyl homogalacturonan pectin from the H. rhamnoides berry-a compound that acts as a potential immunostimulant and anticancer adjuvant. (C) 2015 Elsevier Ltd. All rights reserved.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/47836
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Hailiang,Gao, Tingting,Du, Yuzhi,et al. Anticancer and immunostimulating activities of a novel homogalacturonan from Hippophae rhamnoides L. berry[J]. CARBOHYDRATE POLYMERS,2015.
APA	Wang, Hailiang.,Gao, Tingting.,Du, Yuzhi.,Yang, Hongxia.,Wei, Lixin.,...&Ni, Weihua.(2015).Anticancer and immunostimulating activities of a novel homogalacturonan from Hippophae rhamnoides L. berry.CARBOHYDRATE POLYMERS.
MLA	Wang, Hailiang,et al."Anticancer and immunostimulating activities of a novel homogalacturonan from Hippophae rhamnoides L. berry".CARBOHYDRATE POLYMERS (2015).