Accurate Determination of Amino Acids in Serum Samples by Liquid Chromatography-Tandem Mass Spectrometry Using a Stable Isotope Labeling Strategy

NWIPB OpenIR

	Accurate Determination of Amino Acids in Serum Samples by Liquid Chromatography-Tandem Mass Spectrometry Using a Stable Isotope Labeling Strategy
	Song, Cuihua ; Zhang, Shijuan ; Ji, Zhongyin ; Li, Yipeng ; You, Jinmao
	2015-10-01
发表期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE
摘要	An accurate and sensitive liquid chromatography-tandem mass spectrometry method was developed for the analysis of amino acids (isoleucine, leucine, valine, tyrosine, phenylalanine and tryptophan) in serum samples using a stable isotope labeling strategy. Amino acid samples and standards were, respectively, derivatized by 10-methyl-acridone-2-sulfonyl chloride (d(0)-MASC) and its deuterated counterpart d(3)-MASC to form isotopic pairs which co-eluted and were detected by an MS detector at the same time. Accurate internal standard-based quantification was thereby achieved without the use of any internal standard analogy. The labeling reaction of MASC with amino acids is fast, simple and robust. Besides, derivatization increased the molecular weight of amino acids, and therefore they were shifted out of the background noise which was often observed in low mass region. The instrument LODs were in the range of 1.0-2.5 nmol/L. Linearities calculated by comparing theoretical peak area ratios of d(0)-/d(3)-MASC derivatives with the experimental peak area ratios were excellent with correlation coefficients of >0.995. The proposed method was successfully applied to the analysis of amino acids in serum samples with high sensitivity and accuracy.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/48239
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Song, Cuihua,Zhang, Shijuan,Ji, Zhongyin,et al. Accurate Determination of Amino Acids in Serum Samples by Liquid Chromatography-Tandem Mass Spectrometry Using a Stable Isotope Labeling Strategy[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2015.
APA	Song, Cuihua,Zhang, Shijuan,Ji, Zhongyin,Li, Yipeng,&You, Jinmao.(2015).Accurate Determination of Amino Acids in Serum Samples by Liquid Chromatography-Tandem Mass Spectrometry Using a Stable Isotope Labeling Strategy.JOURNAL OF CHROMATOGRAPHIC SCIENCE.
MLA	Song, Cuihua,et al."Accurate Determination of Amino Acids in Serum Samples by Liquid Chromatography-Tandem Mass Spectrometry Using a Stable Isotope Labeling Strategy".JOURNAL OF CHROMATOGRAPHIC SCIENCE (2015).