Sensitive and background-free determination of thiols from wastewater samples by MOF-5 extraction coupled with high-performance liquid chromatography with fluorescence detection using a novel fluorescence probe of carbazole-9-ethyl-2-maleimide

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	Sensitive and background-free determination of thiols from wastewater samples by MOF-5 extraction coupled with high-performance liquid chromatography with fluorescence detection using a novel fluorescence probe of carbazole-9-ethyl-2-maleimide
	Lv, Zhengxian ; Sun, Zhiwei ; Song, Cuihua ; Lu, Shuaimin ; Chen, Guang ; You, Jinmao
	2016-12-01
发表期刊	TALANTA
摘要	A sensitive and background-free pre-column derivatization method for the determination of thiol compounds using metal-organic framework material (MOF-5) as dispersive solid-phase extraction (DSPE) adsorbent followed by high-performance liquid chromatography fluorescence detection (HPLC-FLD) has been developed. In this paper, a novel labeling reagent, carbazole-9-ethyl-2-maleimide(CAEM), was synthesized and reacted with thiols at 40 degrees C for 10 min in the presence of PBS buffer (0.02 mol/L, pH 7.5). Interestingly, CAEM itself had no fluorescence, while its derivatives exhibited intense fluorescence with an excitation maximum at lambda(ex) 274 nm and an emission maximum at lambda(em) 363 nm, which greatly reduced the background interference and improved the sensitivity of the method. Furthermore, the MOF-5 was prepared and used as DSPE adsorbent for the selective adsorption of thiols from wastewater sample. Under the optimized experimental conditions, an excellent linearity for all analytes over their concentration ranges of 0.01-1.0 mu mol/L (R-2 > 0.9986)were obtained with the limit of detection (LOD) ranging from 8 to 17.1 pmol/L for nine tested thiols. The feasibility of this method for the determination of thiols in wastewater samples had beep evaluated and satisfactory average recoveries (n=3) were achieved with the range of 86.6-98.5%. (C) 2016 Elsevier B.V. All rights reserved.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/48349
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Lv, Zhengxian,Sun, Zhiwei,Song, Cuihua,et al. Sensitive and background-free determination of thiols from wastewater samples by MOF-5 extraction coupled with high-performance liquid chromatography with fluorescence detection using a novel fluorescence probe of carbazole-9-ethyl-2-maleimide[J]. TALANTA,2016.
APA	Lv, Zhengxian,Sun, Zhiwei,Song, Cuihua,Lu, Shuaimin,Chen, Guang,&You, Jinmao.(2016).Sensitive and background-free determination of thiols from wastewater samples by MOF-5 extraction coupled with high-performance liquid chromatography with fluorescence detection using a novel fluorescence probe of carbazole-9-ethyl-2-maleimide.TALANTA.
MLA	Lv, Zhengxian,et al."Sensitive and background-free determination of thiols from wastewater samples by MOF-5 extraction coupled with high-performance liquid chromatography with fluorescence detection using a novel fluorescence probe of carbazole-9-ethyl-2-maleimide".TALANTA (2016).