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Efficient separation of high-purity compounds from Oxytropis falcata using two-dimensional preparative chromatography
Wang, Weidong ; Tao, Yanduo ; Jiao, Lijin ; Fan, Minxia ; Shao, Yun ; Wang, Qilan ; Mei, Lijuan ; Dang, Jun
2017-09-01
Source PublicationJOURNAL OF SEPARATION SCIENCE
AbstractThe separation of high-purity compounds from traditional Tibetan medicines plays an important role in investigating their bioactivity. Nevertheless, it is often quite difficult to isolate compounds with high purity because of the complexity of traditional Tibetan medicines. In this work, an offline two-dimensional reversed-phase preparative method was successfully developed for the separation of high-purity compounds from Oxytropis falcata. Based on the analysis results, an ODS C18 prep column was used for first-dimensional preparation, and 14.8 g of the crude sample was separated into five fractions with a recovery of 74.6%. Then, an XAqua C18 prep column was used to isolate high-purity compounds in the second-dimensional preparation because its separation selectivity is different with the ODS C18 stationary phase. As a result, eight compounds in the crude sample were isolated in more than 98% purity. This is the first report of trans-cinnamic acid (1) and trifolirhizin (2) from Oxytropis falcata. This method has the potential to be an efficient separation method of high-purity compounds from Oxytropis falcata and it shows great promise for the separation of high-purity compounds from complex samples.
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/48657
Collection中国科学院西北高原生物研究所
Recommended Citation
GB/T 7714
Wang, Weidong,Tao, Yanduo,Jiao, Lijin,et al. Efficient separation of high-purity compounds from Oxytropis falcata using two-dimensional preparative chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2017.
APA Wang, Weidong.,Tao, Yanduo.,Jiao, Lijin.,Fan, Minxia.,Shao, Yun.,...&Dang, Jun.(2017).Efficient separation of high-purity compounds from Oxytropis falcata using two-dimensional preparative chromatography.JOURNAL OF SEPARATION SCIENCE.
MLA Wang, Weidong,et al."Efficient separation of high-purity compounds from Oxytropis falcata using two-dimensional preparative chromatography".JOURNAL OF SEPARATION SCIENCE (2017).
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