NWIPB OpenIR
Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization
Ma, Tao ; Hu, Na ; Zhang, Qiulong ; Ding, Chenxi ; Che, Guodong ; Wu, Hongliang ; Sun, Yanan ; Ji, Zhongyin ; You, Jinmao ; Suo, Yourui ; Ding, Chenxu
2018
发表期刊JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
摘要A rapid and sensitive reversed-phase high performance liquid chromatography- fluorescence-mass spectrometer (RP-HPLC-FLD-MS) method based on pre-column derivatization using 2-(5-benzoacridine)ethyl-p-toluenesulfonate (BAETS) as labelling reagent has been developed for simultaneous determination of five triterpenic acids (asiatic acid (AA), maslinic acid (MA), corosolic acid (CA), oleanolic acid (OA), and betulinic acid (BA). The presented method was validated for linearity (correlation coefficient R-2>0.9994), precision (RSD 2.1%-3.9%) and reproducibility (RSD 0.01%-2.1%). The limits of detection (LODs) and the limits of quantitation LOQs were within the range of 0.71-1.02 ng mL(-1) and 2.28-3.24 ng mL(-1), respectively. The proposed method was successfully applied to simultaneously determine five triterpenic acids of four Corydalis plants and showed satisfactory reproducibility and credibility. Moreover, several main parameters affecting extraction procedure and derivatization efficiency were investigated by response surface methodology (RSM), respectively. Triterpenic acid content in four Corydalis plants was measured according to the established method and the results indicated that triterpenic acid contents were various in different organs and herbs.
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/49280
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Ma, Tao,Hu, Na,Zhang, Qiulong,et al. Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2018.
APA Ma, Tao.,Hu, Na.,Zhang, Qiulong.,Ding, Chenxi.,Che, Guodong.,...&Ding, Chenxu.(2018).Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES.
MLA Ma, Tao,et al."Simultaneous determination of five triterpenic acids in four Corydalis herb medicines by reversed-phase high performance liquid chromatography-fluorescence-mass spectrometer (RP-HPLC-FLD-MS) based on pre-column derivatization".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES (2018).
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