Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection

NWIPB OpenIR

	Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection
	You, Jinmao ; Zhu, Fang ; Zhao, Wenchen ; Zhao, Xian'en ; Suo, Yourui ; Liu, Sujuan
	2007-03-01
发表期刊	EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY ; You Jinmao,Zhu Fang,Zhao Wenchen,Zhao Xian'en,Suo Yourui,Liu Sujuan.Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY,2007,109(3):225-236
摘要	A sensitive method for the determination of free fatty acids using 2-(2-(anthracen-10-yl)-1H-naphtho[2,3-dimidazol-1-yl) ethyl-p-toluenesuIfonate (ANITS) as tagging reagent with fluorescence detection has been developed. ANITS could easily and quickly label fatty acids in the presence of the K2CO3 catalyst at 90 degrees C for 40 min in N,N-dimethylformamide solvent. From the extracts of rape bee pollen samples, 20 free fatty acids were sensitively determined. Fatty acid derivatives were separated on a reversed-phase Eclipse XDB-C8 column by HPLC in conjunction with gradient elution. The corresponding derivatives were identified by post-column APCI/MS in positive-ion detection mode. ANITS-fatty acid derivatives gave an intense molecular ion peak at mlz [M+H](+); with MS/MS analysis, the collision-induced dissociation spectra of m/z [M+H](+) produced the specific fragment ions at mlz [M-345](+) and mlz 345.0 (here, m/z 345 is the core structural moiety of the ANITS molecule). The fluorescence excitation and emission wavelengths of the derivatives were lambda(ex) = 250 nm and lambda(em) = 512 nm, respectively. Linear correlation coefficients for all fatty acid derivatives are > 0.9999. Detection limits, at a signal-to-noise ratio of 3 : 1, are 24.76-98.79 fmol for the labeled fatty acids.; A sensitive method for the determination of free fatty acids using 2-(2-(anthracen-10-yl)-1H-naphtho[2,3-dimidazol-1-yl) ethyl-p-toluenesuIfonate (ANITS) as tagging reagent with fluorescence detection has been developed. ANITS could easily and quickly label fatty acids in the presence of the K2CO3 catalyst at 90 degrees C for 40 min in N,N-dimethylformamide solvent. From the extracts of rape bee pollen samples, 20 free fatty acids were sensitively determined. Fatty acid derivatives were separated on a reversed-phase Eclipse XDB-C8 column by HPLC in conjunction with gradient elution. The corresponding derivatives were identified by post-column APCI/MS in positive-ion detection mode. ANITS-fatty acid derivatives gave an intense molecular ion peak at mlz [M+H](+); with MS/MS analysis, the collision-induced dissociation spectra of m/z [M+H](+) produced the specific fragment ions at mlz [M-345](+) and mlz 345.0 (here, m/z 345 is the core structural moiety of the ANITS molecule). The fluorescence excitation and emission wavelengths of the derivatives were lambda(ex) = 250 nm and lambda(em) = 512 nm, respectively. Linear correlation coefficients for all fatty acid derivatives are > 0.9999. Detection limits, at a signal-to-noise ratio of 3 : 1, are 24.76-98.79 fmol for the labeled fatty acids.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/50763
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	You, Jinmao,Zhu, Fang,Zhao, Wenchen,et al. Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection[J]. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, You Jinmao,Zhu Fang,Zhao Wenchen,Zhao Xian'en,Suo Yourui,Liu Sujuan.Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY,2007,109(3):225-236,2007.
APA	You, Jinmao,Zhu, Fang,Zhao, Wenchen,Zhao, Xian'en,Suo, Yourui,&Liu, Sujuan.(2007).Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection.EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY.
MLA	You, Jinmao,et al."Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection".EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY (2007).