Development and characterization of novel microsatellite markers isolated from Potentilla fruticosa L. (Rosaceae), and cross-species amplification in its sister species-Potentilla glabra L.

NWIPB OpenIR

	Development and characterization of novel microsatellite markers isolated from Potentilla fruticosa L. (Rosaceae), and cross-species amplification in its sister species-Potentilla glabra L.
	Wang, Liu-Yang ; Kou, Yi-Xuan ; Wu, Gui-Li ; Wang, Yu-Jin
	2009-12-01
发表期刊	CONSERVATION GENETICS RESOURCES ; Wang, LY; Kou, YX; Wu, GL; Wang, YJ.Development and characterization of novel microsatellite markers isolated from Potentilla fruticosa L. (Rosaceae), and cross-species amplification in its sister species-Potentilla glabra L.,CONSERVATION GENETICS RESOURCES,2009,1(1):51-53
摘要	Here, we report development and characterization of 11 new polymorphic microsatellite loci isolated from the genome of Potentilla fruticosa L. (Rosaceae). Polymorphisms of each locus were screened in 12-15 individuals from geographically separated populations. The number of alleles per locus ranged from five to eleven. The observed and expected heterozygosity ranged from 0.22 to 0.35 and 0.38 to 0.53, respectively. These eleven loci were also surveyed for 11 individuals from its sister species, Potentilla glabra L. (Rosaceae). Ten of them were successfully amplified and allelic diversities were also highly polymorphic. Seven loci totally in both species exhibited significant departure from Hardy-Weinberg equilibrium probably due to excessive homozygosity and the population structure within samples. No linkage disequilibrium was detected among all paired loci comparisons. These results demonstrate that these microsatellite markers can be used to characterise the intra-and inter-specific genetic diversity, and will be useful to study the population genetics and speciation history of the two closely related shrubs.; Here, we report development and characterization of 11 new polymorphic microsatellite loci isolated from the genome of Potentilla fruticosa L. (Rosaceae). Polymorphisms of each locus were screened in 12-15 individuals from geographically separated populations. The number of alleles per locus ranged from five to eleven. The observed and expected heterozygosity ranged from 0.22 to 0.35 and 0.38 to 0.53, respectively. These eleven loci were also surveyed for 11 individuals from its sister species, Potentilla glabra L. (Rosaceae). Ten of them were successfully amplified and allelic diversities were also highly polymorphic. Seven loci totally in both species exhibited significant departure from Hardy-Weinberg equilibrium probably due to excessive homozygosity and the population structure within samples. No linkage disequilibrium was detected among all paired loci comparisons. These results demonstrate that these microsatellite markers can be used to characterise the intra-and inter-specific genetic diversity, and will be useful to study the population genetics and speciation history of the two closely related shrubs.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/51121
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Liu-Yang,Kou, Yi-Xuan,Wu, Gui-Li,et al. Development and characterization of novel microsatellite markers isolated from Potentilla fruticosa L. (Rosaceae), and cross-species amplification in its sister species-Potentilla glabra L.[J]. CONSERVATION GENETICS RESOURCES, Wang, LY; Kou, YX; Wu, GL; Wang, YJ.Development and characterization of novel microsatellite markers isolated from Potentilla fruticosa L. (Rosaceae), and cross-species amplification in its sister species-Potentilla glabra L.,CONSERVATION GENETICS RESOURCES,2009,1(1):51-53,2009.
APA	Wang, Liu-Yang,Kou, Yi-Xuan,Wu, Gui-Li,&Wang, Yu-Jin.(2009).Development and characterization of novel microsatellite markers isolated from Potentilla fruticosa L. (Rosaceae), and cross-species amplification in its sister species-Potentilla glabra L..CONSERVATION GENETICS RESOURCES.
MLA	Wang, Liu-Yang,et al."Development and characterization of novel microsatellite markers isolated from Potentilla fruticosa L. (Rosaceae), and cross-species amplification in its sister species-Potentilla glabra L.".CONSERVATION GENETICS RESOURCES (2009).