Knowledge Management System of Northwest Institute of Plateau Biology, CAS
An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae) | |
Hu, Yanping ; Xie, Xiaolong ; Wang, Li ; Yang, Jian ; Zhang, Huaigang ; Li, Yi | |
2009-06-17 | |
发表期刊 | AFRICAN JOURNAL OF BIOTECHNOLOGY ; Hu, YP; Xie, XL; Wang, L; Yang, J; Zhang, HG; Li, Y.An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae),AFRICAN JOURNAL OF BIOTECHNOLOGY,2009,8(12):2691-2694 |
摘要 | Rhubarb is an important traditional Chinese herbal drug with high secondary metabolites that interfere with DNA extraction procedures and downstream applications, such as DNA restriction and amplification. An effective and low-cost protocol for isolating genomic DNA from root of Rheum tanguticum is described in this paper. It involved a modified CTAB method with distilled water pretreating samples. The A(260)/A(280) absorbance ratio of extracted DNA, free from polysaccharide, polyphenols and tannins contaminants, ranged from 1.85 to 2.0 within the high level of purity. The DNA preparations were found suitable for analyses with restriction enzyme digestion and inter-simple sequence repeats (ISSR). This method is helpful for further studies on rhubarb at molecular level and is more cost-effective than commercial kit.; Rhubarb is an important traditional Chinese herbal drug with high secondary metabolites that interfere with DNA extraction procedures and downstream applications, such as DNA restriction and amplification. An effective and low-cost protocol for isolating genomic DNA from root of Rheum tanguticum is described in this paper. It involved a modified CTAB method with distilled water pretreating samples. The A(260)/A(280) absorbance ratio of extracted DNA, free from polysaccharide, polyphenols and tannins contaminants, ranged from 1.85 to 2.0 within the high level of purity. The DNA preparations were found suitable for analyses with restriction enzyme digestion and inter-simple sequence repeats (ISSR). This method is helpful for further studies on rhubarb at molecular level and is more cost-effective than commercial kit. |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/51148 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Hu, Yanping,Xie, Xiaolong,Wang, Li,et al. An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY, Hu, YP; Xie, XL; Wang, L; Yang, J; Zhang, HG; Li, Y.An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae),AFRICAN JOURNAL OF BIOTECHNOLOGY,2009,8(12):2691-2694,2009. |
APA | Hu, Yanping,Xie, Xiaolong,Wang, Li,Yang, Jian,Zhang, Huaigang,&Li, Yi.(2009).An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae).AFRICAN JOURNAL OF BIOTECHNOLOGY. |
MLA | Hu, Yanping,et al."An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)".AFRICAN JOURNAL OF BIOTECHNOLOGY (2009). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论