Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae)

NWIPB OpenIR

	Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae)
	Li, Ke-xin ; Geng, Jia-ning ; Yang, Jin ; Zhang, Yan-ming ; Hu, Song-nian
	2009-06-01
发表期刊	CONSERVATION GENETICS ; Li, KX; Geng, JN; Yang, J; Zhang, YM; Hu, SN.Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae),CONSERVATION GENETICS,2009,10(3):785-787
摘要	High quality jumping microsatellite libraries of the plateau pika (Ochotona curzoniae) were constructed; 231 out of the 288 clones contained microsatellite repeat motif and 120 pairs of primers were designed accordingly. Polymorphism was assessed for 48 individuals. Only 13 microsatellite loci were polymorphic with high polymorphism information content (PIC) ranging from 0.598 to 0.871, a condition probably resulting from individuals used for assessment being very closely related. This degree of polymorphism, however, is sufficient to conduct parentage analysis. Expected and observed heterozygosities ranged from 0.219 to 0.935 and 0.659 to 0.890, respectively. One locus was in linkage disequilibrium. This information provides efficient tools to allow future parentage studies.; High quality jumping microsatellite libraries of the plateau pika (Ochotona curzoniae) were constructed; 231 out of the 288 clones contained microsatellite repeat motif and 120 pairs of primers were designed accordingly. Polymorphism was assessed for 48 individuals. Only 13 microsatellite loci were polymorphic with high polymorphism information content (PIC) ranging from 0.598 to 0.871, a condition probably resulting from individuals used for assessment being very closely related. This degree of polymorphism, however, is sufficient to conduct parentage analysis. Expected and observed heterozygosities ranged from 0.219 to 0.935 and 0.659 to 0.890, respectively. One locus was in linkage disequilibrium. This information provides efficient tools to allow future parentage studies.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/51156
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, Ke-xin,Geng, Jia-ning,Yang, Jin,et al. Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae)[J]. CONSERVATION GENETICS, Li, KX; Geng, JN; Yang, J; Zhang, YM; Hu, SN.Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae),CONSERVATION GENETICS,2009,10(3):785-787,2009.
APA	Li, Ke-xin,Geng, Jia-ning,Yang, Jin,Zhang, Yan-ming,&Hu, Song-nian.(2009).Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae).CONSERVATION GENETICS.
MLA	Li, Ke-xin,et al."Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae)".CONSERVATION GENETICS (2009).