Application of 10-ethyl-acridine-3-sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI-MS

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	Application of 10-ethyl-acridine-3-sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI-MS
	You, Jinmao ; Zhao, Huaixin ; Sun, Zhiwei ; Xia, Lian ; Yan, Tao ; Suo, Yourui ; Li, Yulin
	2009-05-01
发表期刊	JOURNAL OF SEPARATION SCIENCE ; You, JM; Zhao, HX; Sun, ZW; Xia, L; Yan, T; Suo, YR; Li, YL.Application of 10-ethyl-acridine-3-sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI-MS,JOURNAL OF SEPARATION SCIENCE,2009,32(9):1351-1362
摘要	A simple, sensitive method for the determination of aliphatic amines based on a sulfonylation reaction using 10-ethyl-acridine-3-sulfonyl chloride (EASC) as pre-column labeling reagent with fluorescence detection and APCI-MS identification has been developed. The labeled derivatives exhibited high stability and were enough to be efficiently analyzed by HPLC with an excitation maximum at lambda(ex) 270 nm and an emission maximum at lambda(em) 430 nm. Identification of derivatives was carried out by online post-column MS in positive-ion mode. Comparing with the widely used 5-dimethylaminonaphthalene-1-sulfonylchloride (Dansyl-Cl), EASC-amine derivatives not only exhibited high fluorescence but also exhibited excellent MS ionizable potential. Detection limits obtained from 0.10 pmol injection, at a S/N of 3, were 4.0-12.7 fmol. The mean intra- and inter-assay precision for all aliphatic amine levels were <3.84 and 3.21%, respectively. Excellent linear responses were observed with coefficients of >0.9995.; A simple, sensitive method for the determination of aliphatic amines based on a sulfonylation reaction using 10-ethyl-acridine-3-sulfonyl chloride (EASC) as pre-column labeling reagent with fluorescence detection and APCI-MS identification has been developed. The labeled derivatives exhibited high stability and were enough to be efficiently analyzed by HPLC with an excitation maximum at lambda(ex) 270 nm and an emission maximum at lambda(em) 430 nm. Identification of derivatives was carried out by online post-column MS in positive-ion mode. Comparing with the widely used 5-dimethylaminonaphthalene-1-sulfonylchloride (Dansyl-Cl), EASC-amine derivatives not only exhibited high fluorescence but also exhibited excellent MS ionizable potential. Detection limits obtained from 0.10 pmol injection, at a S/N of 3, were 4.0-12.7 fmol. The mean intra- and inter-assay precision for all aliphatic amine levels were <3.84 and 3.21%, respectively. Excellent linear responses were observed with coefficients of >0.9995.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/51159
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	You, Jinmao,Zhao, Huaixin,Sun, Zhiwei,et al. Application of 10-ethyl-acridine-3-sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI-MS[J]. JOURNAL OF SEPARATION SCIENCE, You, JM; Zhao, HX; Sun, ZW; Xia, L; Yan, T; Suo, YR; Li, YL.Application of 10-ethyl-acridine-3-sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI-MS,JOURNAL OF SEPARATION SCIENCE,2009,32(9):1351-1362,2009.
APA	You, Jinmao.,Zhao, Huaixin.,Sun, Zhiwei.,Xia, Lian.,Yan, Tao.,...&Li, Yulin.(2009).Application of 10-ethyl-acridine-3-sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI-MS.JOURNAL OF SEPARATION SCIENCE.
MLA	You, Jinmao,et al."Application of 10-ethyl-acridine-3-sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI-MS".JOURNAL OF SEPARATION SCIENCE (2009).