Sparation and Preparation of Gentiopicroside and Sweroside from the Extracts of Swertia franchetiana H.Smith by HSCCC

NWIPB OpenIR

	Sparation and Preparation of Gentiopicroside and Sweroside from the Extracts of Swertia franchetiana H.Smith by HSCCC
	CHENG Hui-yun ; TONG LI ; Du YZ(杜玉枝) ; CHEN Xiang-hong ; YUN Hai-xia
	2010-01-10
发表期刊	MEDICINAL PLANT ; CHENG Hui-yun;TONG LI;杜玉枝;CHEN Xiang-hong;YUN Hai-xia.Sparation and Preparation of Gentiopicroside and Sweroside from the Extracts of Swertia franchetiana H.Smith by HSCCC,MEDICINAL PLANT,2010,1(10):38-40
摘要	Objective: To separate and prepare gentiopicroside and sweroside from the extracts of Swertia franchetiana H.Smith by high speed counter-current chromatography (HSCCC). Method: Chloroform: menthanol: water = 4:4:2 (v/v/v) as solvent system, the upper one was stationary phase while the lower one was mobile phase; ultrasonic degassed for 20min; the rotating velocity of 850 r/min; mobile phase flow rate of 2ml/min; the detective wavelength of 254 nm; the purity of product was identified by polarimeter. Result: 14 mg gentiopricroside and 27 mg swersoside were obtained from 100 mg crude extracts of Swertia franchetiana H.mith, the specific rotation [a]tD of which were -196.3°and -126.6°respectively, which suggested the products were of high purity. Conclusion: The study would lay a feasible way for the large scale preparation of gentiopicroside and sweroside by HSCCC.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/51332
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	CHENG Hui-yun,TONG LI,Du YZ,et al. Sparation and Preparation of Gentiopicroside and Sweroside from the Extracts of Swertia franchetiana H.Smith by HSCCC[J]. MEDICINAL PLANT, CHENG Hui-yun;TONG LI;杜玉枝;CHEN Xiang-hong;YUN Hai-xia.Sparation and Preparation of Gentiopicroside and Sweroside from the Extracts of Swertia franchetiana H.Smith by HSCCC,MEDICINAL PLANT,2010,1(10):38-40,2010.
APA	CHENG Hui-yun,TONG LI,杜玉枝,CHEN Xiang-hong,&YUN Hai-xia.(2010).Sparation and Preparation of Gentiopicroside and Sweroside from the Extracts of Swertia franchetiana H.Smith by HSCCC.MEDICINAL PLANT.
MLA	CHENG Hui-yun,et al."Sparation and Preparation of Gentiopicroside and Sweroside from the Extracts of Swertia franchetiana H.Smith by HSCCC".MEDICINAL PLANT (2010).