Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.) | |
Lei, Yunting ; Zhao, Yanyan ; Yu, Feng ; Li, Yuan ; Dou, Quanwen | |
2014-12-01 | |
发表期刊 | CONSERVATION GENETICS RESOURCES ; Lei, YT; Zhao, YY; Yu, F; Li, Y; Dou, QW.Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.),CONSERVATION GENETICS RESOURCES,2014,6(4):861 |
摘要 | Siberian wildrye, Elymus sibiricus L., is an important perennial forage crop in temperate regions of the world, especially in the Qinghai-Tibet plateau. However, it is now threatened by climate warming, excessive grazing, and damage to its natural habitat. A total of 53 polymorphic genomic-SSR markers were isolated and characterized in E. sibiricus. The number of alleles for these markers in 11 individuals varied from 2 to 5, with an average of 3.09 alleles per locus. Segregation analysis in an F-2 population revealed that the tested genomic-SSR markers had a co-dominant nature and Mendelian inheritance. Cross-species amplification tests showed that about half of the total E. sibiricus genomic-SSR markers could be effectively amplified in E. nutans. These SSR markers are the first characterized in E. sibiricus and will be useful for investigating genetic diversity and molecular-assisted breeding.; Siberian wildrye, Elymus sibiricus L., is an important perennial forage crop in temperate regions of the world, especially in the Qinghai-Tibet plateau. However, it is now threatened by climate warming, excessive grazing, and damage to its natural habitat. A total of 53 polymorphic genomic-SSR markers were isolated and characterized in E. sibiricus. The number of alleles for these markers in 11 individuals varied from 2 to 5, with an average of 3.09 alleles per locus. Segregation analysis in an F-2 population revealed that the tested genomic-SSR markers had a co-dominant nature and Mendelian inheritance. Cross-species amplification tests showed that about half of the total E. sibiricus genomic-SSR markers could be effectively amplified in E. nutans. These SSR markers are the first characterized in E. sibiricus and will be useful for investigating genetic diversity and molecular-assisted breeding. |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/52580 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Lei, Yunting,Zhao, Yanyan,Yu, Feng,et al. Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.)[J]. CONSERVATION GENETICS RESOURCES, Lei, YT; Zhao, YY; Yu, F; Li, Y; Dou, QW.Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.),CONSERVATION GENETICS RESOURCES,2014,6(4):861,2014. |
APA | Lei, Yunting,Zhao, Yanyan,Yu, Feng,Li, Yuan,&Dou, Quanwen.(2014).Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.).CONSERVATION GENETICS RESOURCES. |
MLA | Lei, Yunting,et al."Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.)".CONSERVATION GENETICS RESOURCES (2014). |
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