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Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry
Zhao Xian-En ; Lu Tao ; Wei Na ; Zhu Shu-Yun ; You Jin-Mao
2014-11-01
发表期刊CHINESE JOURNAL OF ANALYTICAL CHEMISTRY ; Zhao, XE; Lu, T; Wei, N; Zhu, SY; You, JM.Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry,CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2014,42(11):1629
摘要Commercial Rhodamine B was first reported as a derivatization reagent labeling hydroxyl compound for mass spectrometry sensitization. In virtue of the strategy of ultrasonic-assisted dispersive liquid-liquid microextraction (UA-DLLME) combined with Rhodamine B derivatization, a new method of ultrahigh performance liquid chromatography triple quadrupole mass spectrometry (UHPLC-MS/MS) was developed for the sensitive determination of Panaxadiol (PD). UA-DLLME and derivatization conditions were investigated. PD in cosmetics and ginseng was extracted by UA-DLLME (150 mu L chloroform as extraction solvent, 500 mu L ethanol as disperser solvent, 3 min). Using Rhodamine B as precolumn derivatization reagent, the stable derivative of PD was obtained in acetonitrile at 70 degrees C for 30 min under microwave radiation (800 W) with the catalyst of N, N'-carbonyldiimidazole (CDI) and 4-dimethylaminopyridine (DMAP). And then the PD derivative was separated and detected within 3 min by multiple reaction monitoring (MRM) mode of UHPLC-MS/MS. The LOD was 4.0 ng L-1, and the LOQ was 15.0 ng L-1. This method had perfect linearity, precision and recovery results, and showed obvious advantages such as sensitivity and low matrix effect in comparison with the previously reported methods.; Commercial Rhodamine B was first reported as a derivatization reagent labeling hydroxyl compound for mass spectrometry sensitization. In virtue of the strategy of ultrasonic-assisted dispersive liquid-liquid microextraction (UA-DLLME) combined with Rhodamine B derivatization, a new method of ultrahigh performance liquid chromatography triple quadrupole mass spectrometry (UHPLC-MS/MS) was developed for the sensitive determination of Panaxadiol (PD). UA-DLLME and derivatization conditions were investigated. PD in cosmetics and ginseng was extracted by UA-DLLME (150 mu L chloroform as extraction solvent, 500 mu L ethanol as disperser solvent, 3 min). Using Rhodamine B as precolumn derivatization reagent, the stable derivative of PD was obtained in acetonitrile at 70 degrees C for 30 min under microwave radiation (800 W) with the catalyst of N, N'-carbonyldiimidazole (CDI) and 4-dimethylaminopyridine (DMAP). And then the PD derivative was separated and detected within 3 min by multiple reaction monitoring (MRM) mode of UHPLC-MS/MS. The LOD was 4.0 ng L-1, and the LOQ was 15.0 ng L-1. This method had perfect linearity, precision and recovery results, and showed obvious advantages such as sensitivity and low matrix effect in comparison with the previously reported methods.
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/52583
专题中国科学院西北高原生物研究所
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GB/T 7714
Zhao Xian-En,Lu Tao,Wei Na,et al. Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, Zhao, XE; Lu, T; Wei, N; Zhu, SY; You, JM.Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry,CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2014,42(11):1629,2014.
APA Zhao Xian-En,Lu Tao,Wei Na,Zhu Shu-Yun,&You Jin-Mao.(2014).Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY.
MLA Zhao Xian-En,et al."Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (2014).
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