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DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION | |
Chen, Xiangming ; Dong, Xiuli ; Suo, Yourui ; You, Jinmao | |
2014-07-03 | |
发表期刊 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
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摘要 | A highly sensitive method for the determination of free fatty acids (FFAs) using high-performance liquid chromatography (HPLC) with fluorescence detection has been developed. Twenty-six FFAs (C5-C30) were derivatized by a novel pre-column derivatization reagent 2-(11H-benzo[a]carbazol-11-yl)-ethyl-4-methyl- benzenesulphonate (BCETS). Derivatives were sufficiently stable to be efficiently analyzed by HPLC. All fatty acid derivatives were separated on a hypersil BDS-C8 column in conjunction with a gradient elution with a good baseline resolution. The identification was carried out by post-column online mass spectrometry with atmospheric pressure chemical ionization (APCI) in positive-ion detection mode. The established method exhibited good selectivity and excellent repeatability. The RSD (n=6) for each fatty acid derivative was <2.8%, and detection limit (at signal-to-noise of 3:1) was 15-64 fmol. The method was used to quantify FFAs in Cortex Lycii sample with satisfactory results.; A highly sensitive method for the determination of free fatty acids (FFAs) using high-performance liquid chromatography (HPLC) with fluorescence detection has been developed. Twenty-six FFAs (C5-C30) were derivatized by a novel pre-column derivatization reagent 2-(11H-benzo[a]carbazol-11-yl)-ethyl-4-methyl- benzenesulphonate (BCETS). Derivatives were sufficiently stable to be efficiently analyzed by HPLC. All fatty acid derivatives were separated on a hypersil BDS-C8 column in conjunction with a gradient elution with a good baseline resolution. The identification was carried out by post-column online mass spectrometry with atmospheric pressure chemical ionization (APCI) in positive-ion detection mode. The established method exhibited good selectivity and excellent repeatability. The RSD (n=6) for each fatty acid derivative was <2.8%, and detection limit (at signal-to-noise of 3:1) was 15-64 fmol. The method was used to quantify FFAs in Cortex Lycii sample with satisfactory results. |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/52618 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Chen, Xiangming,Dong, Xiuli,Suo, Yourui,et al. DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, Chen, XM; Dong, XL; Suo, YR; You, JM.DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION,JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2014,37(11):1548,2014. |
APA | Chen, Xiangming,Dong, Xiuli,Suo, Yourui,&You, Jinmao.(2014).DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES. |
MLA | Chen, Xiangming,et al."DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES (2014). |
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