Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC

NWIPB OpenIR

	Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC
	Chen, Chen ; Zhao, Xiao-Hui ; Yue, Hui-Lan ; Li, Yu-Lin ; Chen, Tao
	2014-05-01
发表期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE ; Chen, C; Zhao, XH; Yue, HL; Li, YL; Chen, T.Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC,JOURNAL OF CHROMATOGRAPHIC SCIENCE,2014,52(5):395
摘要	An effective high-speed counter-current chromatography method was established for the preparative isolation and purification of two phenyl-propanoid glycosides from the Tibetan medicinal plant Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong. With a two-phase solvent system composed of chloroform-n-butanol-methanol-water (4:3:4:5, v/v), 40 mg of an extract of Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong was separated to yield 20 mg of verbascoside and 18 mg of isoacteoside, with purity values of 97 and 98%, respectively. The chemical structures of these two components were identified by proton and carbon nuclear magnetic resonance. In addition, the antioxidant activity of the two phenylpropanoid glycosides was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH)-high-performance liquid chromatography, and the results showed that the two components exhibited strong antioxidant DPPH radical scavenging activity, with IC50 values of 15.6 and 18.9 mg/mL, respectively.; An effective high-speed counter-current chromatography method was established for the preparative isolation and purification of two phenyl-propanoid glycosides from the Tibetan medicinal plant Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong. With a two-phase solvent system composed of chloroform-n-butanol-methanol-water (4:3:4:5, v/v), 40 mg of an extract of Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong was separated to yield 20 mg of verbascoside and 18 mg of isoacteoside, with purity values of 97 and 98%, respectively. The chemical structures of these two components were identified by proton and carbon nuclear magnetic resonance. In addition, the antioxidant activity of the two phenylpropanoid glycosides was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH)-high-performance liquid chromatography, and the results showed that the two components exhibited strong antioxidant DPPH radical scavenging activity, with IC50 values of 15.6 and 18.9 mg/mL, respectively.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/52641
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Chen, Chen,Zhao, Xiao-Hui,Yue, Hui-Lan,et al. Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE, Chen, C; Zhao, XH; Yue, HL; Li, YL; Chen, T.Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC,JOURNAL OF CHROMATOGRAPHIC SCIENCE,2014,52(5):395,2014.
APA	Chen, Chen,Zhao, Xiao-Hui,Yue, Hui-Lan,Li, Yu-Lin,&Chen, Tao.(2014).Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC.JOURNAL OF CHROMATOGRAPHIC SCIENCE.
MLA	Chen, Chen,et al."Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC".JOURNAL OF CHROMATOGRAPHIC SCIENCE (2014).