Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum | |
Chen, Tao ; Liu, Yongling ; Zou, Denglang ; Chen, Chen ; You, Jinmao ; Zhou, Guoying ; Sun, Jing ; Li, Yulin | |
2014 | |
发表期刊 | JOURNAL OF SEPARATION SCIENCE
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摘要 | This study presents an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum. A new solvent system composed of petroleum ether/ethyl acetate/water (4:2:1, v/v/v) was developed for the liquid-liquid extraction of the crude extract from R. tanguticum. As a result, emodin, aloe-emodin, physcion, and chrysophanol were greatly enriched in the organic layer. In addition, an efficient method was successfully established to separate and purify the above anthraquinones by high-speed counter-current chromatography and preparative HPLC. This study supplies a new alternative method for the rapid enrichment, separation, and purification of emodin, aloe-emodin, physcione, and chrysophanol.; This study presents an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum. A new solvent system composed of petroleum ether/ethyl acetate/water (4:2:1, v/v/v) was developed for the liquid-liquid extraction of the crude extract from R. tanguticum. As a result, emodin, aloe-emodin, physcion, and chrysophanol were greatly enriched in the organic layer. In addition, an efficient method was successfully established to separate and purify the above anthraquinones by high-speed counter-current chromatography and preparative HPLC. This study supplies a new alternative method for the rapid enrichment, separation, and purification of emodin, aloe-emodin, physcione, and chrysophanol. |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/52700 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Chen, Tao,Liu, Yongling,Zou, Denglang,et al. Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum[J]. JOURNAL OF SEPARATION SCIENCE, Chen, T; Liu, YL; Zou, DL; Chen, C; You, JM; Zhou, GY; Sun, J; Li, YL.Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum,JOURNAL OF SEPARATION SCIENCE,2014,37(41641):165,2014. |
APA | Chen, Tao.,Liu, Yongling.,Zou, Denglang.,Chen, Chen.,You, Jinmao.,...&Li, Yulin.(2014).Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum.JOURNAL OF SEPARATION SCIENCE. |
MLA | Chen, Tao,et al."Application of an efficient strategy based on liquid-liquid extraction, high-speed counter-current chromatography, and preparative HPLC for the rapid enrichment, separation, and purification of four anthraquinones from Rheum tanguticum".JOURNAL OF SEPARATION SCIENCE (2014). |
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