Rapid, Selective, and Sensitive Analysis of Triterpenic Acids in Hippophae rhamnoides L. Using HPLC with Pre-Column Fluorescent Derivatization and Identification with Post-Column APCI-MS

NWIPB OpenIR

	Rapid, Selective, and Sensitive Analysis of Triterpenic Acids in Hippophae rhamnoides L. Using HPLC with Pre-Column Fluorescent Derivatization and Identification with Post-Column APCI-MS
	Hu, N ; Suo, YR ; Zhang, QL ; You, JM ; Ji, ZY ; Wang, AH ; Han, LJ ; Lv, HH ; Ye, Y ; Suo, YR (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China.
	2015
发表期刊	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
摘要	Hippophae rhamnoides L., a fruit which is rich in natural chemical ingredients, has become an important raw material of health food, medicine, and cosmetics. In the present work, a method using 2-(5-benzoacridine)ethyl-p-toluenesulfonate as pre-column derivatization reagent with high sensitivity, selectivity, and short separation time was developed to analyze triterpenic acids (TTAs) in H. rhamnoides L. by HPLC with fluorescence detection and simultaneously confirmed by post-column atmospheric pressure chemical ionization-mass spectrometry. The method was validated by linearity, limits of detection (LODs) and lower limits of quantification (LLOQs), precision, and accuracy. Good linear correlations were observed for all TTAs with correlation coefficients greater than 0.9996. LODs and LLOQs were in the range of 1.71-2.14 and 7.45-8.23ngmL(-1), respectively. The method was successfully employed to analyze TTAs in H. rhamnoides L. from various origins in the Qinghai-Tibetan plateau with trace amount of samples within 15min. A systemic mapping of the TTAs in H. rhamnoides L. of different origins was established. The results indicated that H. rhamnoides L. is rich in TTAs, especially in oleanolic and ursolic acids, and the content of TTAs varies across origins. This work will support the research on pharmaceutical applications of H. rhamnoides L. and provide information for quality control.; Hippophae rhamnoides L., a fruit which is rich in natural chemical ingredients, has become an important raw material of health food, medicine, and cosmetics. In the present work, a method using 2-(5-benzoacridine)ethyl-p-toluenesulfonate as pre-column derivatization reagent with high sensitivity, selectivity, and short separation time was developed to analyze triterpenic acids (TTAs) in H. rhamnoides L. by HPLC with fluorescence detection and simultaneously confirmed by post-column atmospheric pressure chemical ionization-mass spectrometry. The method was validated by linearity, limits of detection (LODs) and lower limits of quantification (LLOQs), precision, and accuracy. Good linear correlations were observed for all TTAs with correlation coefficients greater than 0.9996. LODs and LLOQs were in the range of 1.71-2.14 and 7.45-8.23ngmL(-1), respectively. The method was successfully employed to analyze TTAs in H. rhamnoides L. from various origins in the Qinghai-Tibetan plateau with trace amount of samples within 15min. A systemic mapping of the TTAs in H. rhamnoides L. of different origins was established. The results indicated that H. rhamnoides L. is rich in TTAs, especially in oleanolic and ursolic acids, and the content of TTAs varies across origins. This work will support the research on pharmaceutical applications of H. rhamnoides L. and provide information for quality control.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/52877
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Hu, N,Suo, YR,Zhang, QL,et al. Rapid, Selective, and Sensitive Analysis of Triterpenic Acids in Hippophae rhamnoides L. Using HPLC with Pre-Column Fluorescent Derivatization and Identification with Post-Column APCI-MS[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2015.
APA	Hu, N.,Suo, YR.,Zhang, QL.,You, JM.,Ji, ZY.,...&Suo, YR .(2015).Rapid, Selective, and Sensitive Analysis of Triterpenic Acids in Hippophae rhamnoides L. Using HPLC with Pre-Column Fluorescent Derivatization and Identification with Post-Column APCI-MS.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES.
MLA	Hu, N,et al."Rapid, Selective, and Sensitive Analysis of Triterpenic Acids in Hippophae rhamnoides L. Using HPLC with Pre-Column Fluorescent Derivatization and Identification with Post-Column APCI-MS".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES (2015).