Dual ultrasonic-assisted dispersive liquid-liquid microextraction coupled with microwave-assisted derivatization for simultaneous determination of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol by ultra high performance liquid chromatography-tandem mass spectrometry

NWIPB OpenIR

	Dual ultrasonic-assisted dispersive liquid-liquid microextraction coupled with microwave-assisted derivatization for simultaneous determination of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol by ultra high performance liquid chromatography-tandem mass spectrometry
	Zhao, Xian-En ; Lv, Tao ; Zhu, Shuyun ; Qu, Fei ; Chen, Guang ; He, Yongrui ; Wei, Na ; Li, Guoliang ; Xia, Lian ; Sun, Zhiwei ; Zhang, Shijuan ; You, Jinmao ; Liu, Shu ; Liu, Zhigiang ; Sun, Jing ; Liu, Shuying
	2016-03-11
发表期刊	JOURNAL OF CHROMATOGRAPHY A
摘要	This paper, for the first time, reported a speedy hyphenated technique of low toxic dual ultrasonic assisted dispersive liquid liquid microextraction (dual-UADLLME) coupled with microwave-assisted derivatization (MAD) for the simultaneous determination of 20(S)-protopanaxadiol (PPD) and 20(S)-protopanaxatriol (PPT). The developed method was based on ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) detection using multiple-reaction monitoring (MRM) mode. A mass spectrometry sensitizing reagent, 4'-carboxy-substituted rosamine (CSR) with high reaction activity and ionization efficiency was synthesized and firstly used as derivatization reagent. Parameters of dual-UADLLME, MAD and UHPLC-MS/MS conditions were all optimized in detail. Low toxic brominated solvents were used as extractant instead of traditional chlorinated solvents. Satisfactory linearity, recovery, repeatability, accuracy and precision, absence of matrix effect and extremely low limits of detection (LODs, 0.010 and 0.015 ng/mL for PPD and PPT, respectively) were achieved. The main advantages were rapid, sensitive and environmentally friendly, and exhibited high selectivity, accuracy and good matrix effect results. The proposed method was successfully applied to pharmacokinetics of PPD and PPT in rat plasma. (C) 2016 Elsevier B.V. All rights reserved.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/53071
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhao, Xian-En,Lv, Tao,Zhu, Shuyun,et al. Dual ultrasonic-assisted dispersive liquid-liquid microextraction coupled with microwave-assisted derivatization for simultaneous determination of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol by ultra high performance liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2016.
APA	Zhao, Xian-En.,Lv, Tao.,Zhu, Shuyun.,Qu, Fei.,Chen, Guang.,...&Liu, Shuying.(2016).Dual ultrasonic-assisted dispersive liquid-liquid microextraction coupled with microwave-assisted derivatization for simultaneous determination of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol by ultra high performance liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY A.
MLA	Zhao, Xian-En,et al."Dual ultrasonic-assisted dispersive liquid-liquid microextraction coupled with microwave-assisted derivatization for simultaneous determination of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol by ultra high performance liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY A (2016).