NWIPB OpenIR
Direct and indirect fluorescent detection of tetracyclines using dually emitting carbon dots
Qu, Fei ; Sun, Zhe ; Liu, Dongya ; Zhao, Xianen ; You, Jinmao
2016-09-01
发表期刊MICROCHIMICA ACTA
摘要The authors describe dual-emission carbon nanodots containing blue emitters (BE; peak emission at 385nm under 315 nm excitation) and yellow emitters (YE; peak emission at 530 nm under 365nm excitation), and how they can be applied to direct and indirect determination of tetracyclines (TCs). The direct detection scheme is based on the finding that tetracycline (TET), oxytetracycline, chlortetracycline and doxycycline quench the two emissions of the carbon dots. While direct determination is rapid and convenient, it cannot differentiate between TCs. The indirect detection scheme, in contrast, is based on the finding that Al (III) ions enhance the fluorescence of the YE in the carbon dots, and that they cause a blue shift in emission. It is, however, known that TET forms a strong complex with Al (III), and this can inhibit the interaction between Al (III) and the YE, so that the fluorescence of YE is not enhanced and blue-shifted by Al (III) in the presence of TET. This finding is exploited in a fluorescence turn-on/off assay for TET that can distinguish TET from other TCs. The linear range of indirect determination for TET extends from 1 nM to 30 mu M, and the limit of detection is 0.52 nM. The indirect method was successfully applied to the determination of TET in spiked milk, fish and pork, and recoveries ranged from 91.7 to 102 %.
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/53234
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Qu, Fei,Sun, Zhe,Liu, Dongya,et al. Direct and indirect fluorescent detection of tetracyclines using dually emitting carbon dots[J]. MICROCHIMICA ACTA,2016.
APA Qu, Fei,Sun, Zhe,Liu, Dongya,Zhao, Xianen,&You, Jinmao.(2016).Direct and indirect fluorescent detection of tetracyclines using dually emitting carbon dots.MICROCHIMICA ACTA.
MLA Qu, Fei,et al."Direct and indirect fluorescent detection of tetracyclines using dually emitting carbon dots".MICROCHIMICA ACTA (2016).
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