Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography

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	Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography
	Yuan, Zhenzhen ; Lv, Huanhuan ; Wang, Xiaoyan ; Li, Gang ; Suo, Yourui ; Zhang, Zhang ; Wang, Honglun
	2016
发表期刊	SEPARATION SCIENCE AND TECHNOLOGY
摘要	Four tannins were isolated and identified from Potentilla parvifolia using high-speed counter-current chromatography (HSCCC) in this study. A two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:11:1.2:11, v/v/v/v) was chosen and yielded 18.8 mg of procyanidin B3, 9.8 mg of procyanidin B6, 9.1 mg of procyanidin B7, and 6.9 mg of 2,3,4,6-tetra-O-galloyl-D-glucose, all at over 95% purity, from 120 mg of the crude sample powder. The identifications were performed with H-1 NMR and C-13 NMR. This is the first report showing the presence of procyanidin B7 and 2,3,4,6-tetra-O-galloyl-D-glucose in the genus Potentilla, and procyanidin B3 and procyanidin B6 were isolated from P. parvifolia for the first time.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/53253
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yuan, Zhenzhen,Lv, Huanhuan,Wang, Xiaoyan,et al. Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography[J]. SEPARATION SCIENCE AND TECHNOLOGY,2016.
APA	Yuan, Zhenzhen.,Lv, Huanhuan.,Wang, Xiaoyan.,Li, Gang.,Suo, Yourui.,...&Wang, Honglun.(2016).Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography.SEPARATION SCIENCE AND TECHNOLOGY.
MLA	Yuan, Zhenzhen,et al."Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography".SEPARATION SCIENCE AND TECHNOLOGY (2016).