Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC

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	Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC
	Luan, Guangxiang ; Wang, Honglun ; Lv, Huanhuan ; Hu, Na ; Suo, Yourui ; Wang, Xiaoyan
	2016-07-01
发表期刊	CHROMATOGRAPHIA
摘要	A method of using high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase liquid chromatography (semi-preparative RPLC) to preparatively separate flavone glucosides and lignan from the crude extracts of Caragana korshinskii has been established for the first time in this study. Five flavone glucosides, including 9 mg of kaempferol 3-O-{beta-d-glucopyranosyl(1 -> 2)-[alpha-l-rhamonopyranosyl(1 -> 6)]-beta-d-galactopyranoside}, 21 mg of kaempferol 3-O-alpha-l-rhamnopyranosyl(1 -> 6)-beta-d-galactopyranoside-7-O-alpha-l-rhamnopyranoside, 34 mg of kaempferol 3-O-beta-d-galactopyranoside-7-O-alpha-l-rhamnopyranoside, 27 mg of kaempferol 3-O-beta-d-glucopyranosyl-7-O-alpha-l-rhamnopyranoside, and 14 mg of calycosin 7-O-beta-d-glucopyranoside, and one lignan, 16 mg of alangilignoside B, were successfully isolated from 1.8 g of the crude sample through the combination of HSCCC with a two-phase solvent system composed of ethyl acetate-n-butanol-0.5 % formic acid (4:1:5, v/v/v) and semi-preparative RPLC with a mobile phase of methanol-water (20:80, v/v). The purities of the six compounds are all over 95 % as determined by HPLC and the structures are confirmed by the analysis of UV, H-1-NMR, and C-13-NMR and compared with published data.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/53281
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Luan, Guangxiang,Wang, Honglun,Lv, Huanhuan,et al. Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC[J]. CHROMATOGRAPHIA,2016.
APA	Luan, Guangxiang,Wang, Honglun,Lv, Huanhuan,Hu, Na,Suo, Yourui,&Wang, Xiaoyan.(2016).Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC.CHROMATOGRAPHIA.
MLA	Luan, Guangxiang,et al."Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC".CHROMATOGRAPHIA (2016).