NWIPB OpenIR
Application of chromatography technology in the separation of active alkaloids from Hypecoum leptocarpum and their inhibitory effect on fatty acid synthase
Zhang, Qiulong ; Luan, Guangxiang ; Ma, Tao ; Hu, Na ; Suo, Yourui ; Wang, Xiaoyan ; Ma, Xiaofeng ; Ding, Chenxu
2015-12-01
发表期刊JOURNAL OF SEPARATION SCIENCE
摘要A method that involved the combination of pH-zone-refining counter-current chromatography and semipreparative reversed-phase liquid chromatography has been established for the preparative separation of alkaloids from Hypecoum leptocarpum. From 1.2 g of crude sample, 31 mg N-feruloyltyramine, 27 mg oxohydrastinine, 47 mg hydroprotopine, 25 mg leptopidine, and 18 mg hypecocarpine have been obtained. The structure of the new compound, hypecocarpine, is confirmed based on the analysis of spectroscopic data, including NMR, UV, and IR spectroscopy and positive electrospray ionization mass spectrometry. The known chemical structures were characterized on the basis of H-1 and C-13 NMR spectroscopy. The purities of the five alkaloids are all over 92.7% as determined by high-performance liquid chromatography. The alkaloids' cytotoxicity in breast cancer cells is assessed by using a Cell Counting Kit assay and their inhibitory effect on fatty acid synthase expression is assessed by a Western blot assay. These results suggest that leptopidine could suppress growth and induce cytotoxicity in breast cancer cells and that the cytotoxicity of leptopidine may be related to its inhibitory effect on fatty acid synthase expression.
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/53303
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Zhang, Qiulong,Luan, Guangxiang,Ma, Tao,et al. Application of chromatography technology in the separation of active alkaloids from Hypecoum leptocarpum and their inhibitory effect on fatty acid synthase[J]. JOURNAL OF SEPARATION SCIENCE,2015.
APA Zhang, Qiulong.,Luan, Guangxiang.,Ma, Tao.,Hu, Na.,Suo, Yourui.,...&Ding, Chenxu.(2015).Application of chromatography technology in the separation of active alkaloids from Hypecoum leptocarpum and their inhibitory effect on fatty acid synthase.JOURNAL OF SEPARATION SCIENCE.
MLA Zhang, Qiulong,et al."Application of chromatography technology in the separation of active alkaloids from Hypecoum leptocarpum and their inhibitory effect on fatty acid synthase".JOURNAL OF SEPARATION SCIENCE (2015).
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