Preparative Separation of N-Feruloyl Serotonin and N-(p-Coumaroyl) Serotonin from Safflower Seed Meal Using High-Speed Counter-Current Chromatography

NWIPB OpenIR

	Preparative Separation of N-Feruloyl Serotonin and N-(p-Coumaroyl) Serotonin from Safflower Seed Meal Using High-Speed Counter-Current Chromatography
	Zhang, Qiulong ; Hu, Na ; Li, Wencong ; Ding, Chenxi ; Ma, Tao ; Bai, Bo ; Wang, Honglun ; Suo, Yourui ; Wang, Xiaoyan ; Ding, Chenxu
	2015-09-01
发表期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE
摘要	High-speed counter-current chromatography (HSCCC) was successfully applied for the preparative separation and purification of N-feruloyl serotonin (NF) and N-(p-coumaroyl) serotonin (NP) from safflower seed meal. After the measurement of partition coefficient of the two target compounds in the two-phase solvent systems, the HSCCC was performed well with a two-phase solvent system composed of CHCl3-methanol-0.1 M HCl at a volume ratio of 1 : 1 : 1, v/v. The upper phase was used as stationary phase and the lower phase was used as mobile phase. Under the optimized condition, 7.5 mg NF and 6.9 mg NP were separated from 40 mg crude sample with the purity of 98.8 and 97.3%, respectively. The structures of the isolated compounds were identified by H-1 NMR and C-13 NMR.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/53316
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhang, Qiulong,Hu, Na,Li, Wencong,et al. Preparative Separation of N-Feruloyl Serotonin and N-(p-Coumaroyl) Serotonin from Safflower Seed Meal Using High-Speed Counter-Current Chromatography[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2015.
APA	Zhang, Qiulong.,Hu, Na.,Li, Wencong.,Ding, Chenxi.,Ma, Tao.,...&Ding, Chenxu.(2015).Preparative Separation of N-Feruloyl Serotonin and N-(p-Coumaroyl) Serotonin from Safflower Seed Meal Using High-Speed Counter-Current Chromatography.JOURNAL OF CHROMATOGRAPHIC SCIENCE.
MLA	Zhang, Qiulong,et al."Preparative Separation of N-Feruloyl Serotonin and N-(p-Coumaroyl) Serotonin from Safflower Seed Meal Using High-Speed Counter-Current Chromatography".JOURNAL OF CHROMATOGRAPHIC SCIENCE (2015).