NWIPB OpenIR
Development of an Efficient HPLC Fluorescence Detection Method for Brassinolide by Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with Derivatization
Lv, Tao ; Zhao, Xian-En ; Zhu, Shuyun ; Ji, Zhongyin ; Chen, Guang ; Sun, Zhiwei ; Song, Cuihua ; You, Jinmao ; Suo, Yourui ; Zhao, XE (reprint author), Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China.
2014-12-01
发表期刊CHROMATOGRAPHIA
摘要A novel hyphenated technique based on ultrasonic-assisted dispersive liquid-liquid microextraction (UA-DLLME) coupled with derivatization has been established for the determination of brassinolide (BL, a representative of brassinosteroids) by HPLC fluorescence detection. 9-Phenanthreneboronic acid is used as labeling reagent of BL. UA-DLLME parameters containing type and volume of extraction and disperser solvent, pH and ultrasonication time are optimized. Derivatization parameters are optimized included amount of 9-phenanthreneboronic acid, volume ratio of pyridine, derivatization time and temperature. Under optimal conditions, quantitative linear range of BL is 50-1,000 ng L-1 and excellent linear response is observed with correlation coefficient of 0.9996. Limit of detection and limit of quantification are calculated as 8.0 and 25.0 ng L-1, respectively. RSDs of retention time and peak area are in the range of 0.68-0.97 % and 4.61-6.54 % for intra-day precision, 1.32-1.94 % and 7.28-9.75 % for inter-day precision, respectively. Accuracy is satisfactory in the range of 82.3-125.1 %. RSDs' values of repeatability are in the range of 0.82-1.79 and 3.95-8.53 % for retention time and peak area, respectively. Enrichment factor for BL is 189. The results of recovery and matrix effect are in the range of 82.0-108.6 and 90.0-115.3 %, respectively. The proposed method has been applied for the determination of BL in Arabidopsis thaliana, Daucus carota and Brassica campestris L. leaves with much higher sensitivity than many other methods.
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/53393
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Lv, Tao,Zhao, Xian-En,Zhu, Shuyun,et al. Development of an Efficient HPLC Fluorescence Detection Method for Brassinolide by Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with Derivatization[J]. CHROMATOGRAPHIA,2014.
APA Lv, Tao.,Zhao, Xian-En.,Zhu, Shuyun.,Ji, Zhongyin.,Chen, Guang.,...&Zhao, XE .(2014).Development of an Efficient HPLC Fluorescence Detection Method for Brassinolide by Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with Derivatization.CHROMATOGRAPHIA.
MLA Lv, Tao,et al."Development of an Efficient HPLC Fluorescence Detection Method for Brassinolide by Ultrasonic-Assisted Dispersive Liquid-Liquid Microextraction Coupled with Derivatization".CHROMATOGRAPHIA (2014).
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