Quantitative iTRAQ Proteomics Revealed Possible Roles for Antioxidant Proteins in Sorghum Aluminum Tolerance

NWIPB OpenIR

	Quantitative iTRAQ Proteomics Revealed Possible Roles for Antioxidant Proteins in Sorghum Aluminum Tolerance
	Zhou, Dangwei ; Yang, Yong ; Zhang, Jinbiao ; Jiang, Fei ; Craft, Eric ; Thannhauser, Theodore W. ; Kochian, Leon V. ; Liu, Jiping
	2017-01-09
发表期刊	FRONTIERS IN PLANT SCIENCE
摘要	Aluminum (Al) toxicity inhibits root growth and limits crop yields on acid soils worldwide. However, quantitative information is scarce on protein expression profiles under Al stress in crops. In this study, we report on the identification of potential Al responsive proteins from root tips of Al sensitive BR007 and Al tolerant SC566 sorghum lines using a strategy employing iTRAQ and 2D-liquid chromatography (LC) coupled to MS/MS (2D-LC-MS/MS). A total of 771 and 329 unique proteins with abundance changes of >1.5 or <0.67-told were identified in BR007 and SC566, respectively. Protein interaction and pathway analyses indicated that proteins involved in the antioxidant system were more abundant in the tolerant line than in the sensitive one after Al treatment, while opposite trends were observed for proteins involved in lignin biosynthesis. Higher levels of ROS accumulation in root tips of the sensitive line due to decreased activity of antioxidant enzymes could lead to higher lignin production and hyper-accumulation of toxic Al in cell walls. These results indicated that activities of peroxidases and the balance between production and consumption of ROS could be important for Al tolerance and lignin biosynthesis in sorghum.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/53425
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhou, Dangwei,Yang, Yong,Zhang, Jinbiao,et al. Quantitative iTRAQ Proteomics Revealed Possible Roles for Antioxidant Proteins in Sorghum Aluminum Tolerance[J]. FRONTIERS IN PLANT SCIENCE,2017.
APA	Zhou, Dangwei.,Yang, Yong.,Zhang, Jinbiao.,Jiang, Fei.,Craft, Eric.,...&Liu, Jiping.(2017).Quantitative iTRAQ Proteomics Revealed Possible Roles for Antioxidant Proteins in Sorghum Aluminum Tolerance.FRONTIERS IN PLANT SCIENCE.
MLA	Zhou, Dangwei,et al."Quantitative iTRAQ Proteomics Revealed Possible Roles for Antioxidant Proteins in Sorghum Aluminum Tolerance".FRONTIERS IN PLANT SCIENCE (2017).