Mass spectrometric identification of aliphatic amines using 2-(11H-Benzo[a] carbazol-11-yl) ethyl chloroformate as fluorescence labeling reagent

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	Mass spectrometric identification of aliphatic amines using 2-(11H-Benzo[a] carbazol-11-yl) ethyl chloroformate as fluorescence labeling reagent
	Zhao Wen-Chen ; Liu Ling-Jun ; Zhu Fang ; Zhao Xian-En ; Liu Su-Juan ; Suo You-Rui ; You Jin-Mao
	2007-09-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
摘要	A simple and highly sensitive method based on the pre-column derivatization for the determination of aliphatic amines in food using a nitrogen -containing 2-( 1 1H-benzo [ a] carbazol-11-yl) ethyl chloroformate BCEC-Cl) as fluorescence labeling reagent followed by high-performance liquid chromatography with fluorescence detection has been developed. The stable derivatives can be obtained by the labeling reaction of BCEC-Cl with amines in the presence of borate buffer ( pH 9. 0) within 3 min. The maximum excitation and emission wavelengths were 279 nm of lambda(ex) and 380 nm of lambda(em) respectively. Separation of 12 amine-derivatives was achieved by a gradient elution on a reversed-phase Eclipse XDB C-8 ( 150 min x 4. 6 mm, 5 mu m) column with a good baseline resolution. The identification of amine -derivatives was carried out by the online post-column MS using atmospheric pressure chemical ionization (APCI) as ion source in positive-Ion detection mode. The established method exhibits excellent reproducibility and recovery. Excellent linear responses were observed with the correlation coefficients > 0. 9996. The detection I units ( at signal-to-noise of 3 : 1) were at levels 1. 8 - 14 fmol.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/53562
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhao Wen-Chen,Liu Ling-Jun,Zhu Fang,et al. Mass spectrometric identification of aliphatic amines using 2-(11H-Benzo[a] carbazol-11-yl) ethyl chloroformate as fluorescence labeling reagent[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2007.
APA	Zhao Wen-Chen.,Liu Ling-Jun.,Zhu Fang.,Zhao Xian-En.,Liu Su-Juan.,...&You Jin-Mao.(2007).Mass spectrometric identification of aliphatic amines using 2-(11H-Benzo[a] carbazol-11-yl) ethyl chloroformate as fluorescence labeling reagent.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY.
MLA	Zhao Wen-Chen,et al."Mass spectrometric identification of aliphatic amines using 2-(11H-Benzo[a] carbazol-11-yl) ethyl chloroformate as fluorescence labeling reagent".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (2007).