NWIPB OpenIR
Genetic transformation of the endangered Tibetan medicinal plant Przewalskia tangutica Maxim and alkaloid production profiling revealed by HPLC
Lei, Tianxiang ; Wang, Huan ; Li, Songling ; Shen, Jianwei ; Chen, Shilong ; Cai, Xiaojian ; Zhou, Dangwei
2018-03-13
发表期刊3 BIOTECH
摘要To achieve a high yield of tropane alkaloids (TA) and exploit the alpine plant sustainably, an optimized protocol for induction and establishment of hairy roots culture of Prezwalskia tangutica Maxim was developed through selection of appropriate Agrobacterium strain and the explant type. The hypocotyl is more readily facile to induce the HR than the cotyledon is when infected with the three different agrobacterium strains. MUS440 has an efficiency (of up to 20%), whereas the ATCC10060 (A4) can induce HR on both types of explants with the highest frequency (33.33%), root length (21.17 +/- 2.84 cm), and root number (10.83 +/- 1.43) per explant than the other strains. The highest HR production resulted from using hypocotyl as explants. Independent transformed HR was able to grow vigorously and to propagate on a no-hormone 1/2MS liquid medium. The presence of pRi rolB gene in transformation of HR was confirmed by PCR amplification. In the liquid medium, the HR growth curve appeared to be "S" shaped, and ADB had increased to 4.633 g/l. Moreover, HPLC analysis showed that HR lines have an extraordinary ability to produce atropine (229.88 mg/100 g), anisodine (4.09 mg/100 g), anisodamine (12.85 mg/100 g), and scopolamine (10.69 mg/100 g), which were all more significant than the control roots. In conclusion, our study optimized the culture condition and established a feasible genetics reactor for P. tangutica green exploration and biological study in the alpine region.
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/54365
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Lei, Tianxiang,Wang, Huan,Li, Songling,et al. Genetic transformation of the endangered Tibetan medicinal plant Przewalskia tangutica Maxim and alkaloid production profiling revealed by HPLC[J]. 3 BIOTECH,2018.
APA Lei, Tianxiang.,Wang, Huan.,Li, Songling.,Shen, Jianwei.,Chen, Shilong.,...&Zhou, Dangwei.(2018).Genetic transformation of the endangered Tibetan medicinal plant Przewalskia tangutica Maxim and alkaloid production profiling revealed by HPLC.3 BIOTECH.
MLA Lei, Tianxiang,et al."Genetic transformation of the endangered Tibetan medicinal plant Przewalskia tangutica Maxim and alkaloid production profiling revealed by HPLC".3 BIOTECH (2018).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lei, Tianxiang]的文章
[Wang, Huan]的文章
[Li, Songling]的文章
百度学术
百度学术中相似的文章
[Lei, Tianxiang]的文章
[Wang, Huan]的文章
[Li, Songling]的文章
必应学术
必应学术中相似的文章
[Lei, Tianxiang]的文章
[Wang, Huan]的文章
[Li, Songling]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。