NWIPB OpenIR
Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology
Wu, Hongliang1,3; Li, Guoliang2,4; Liu, Shucheng4; Ji, Zhongyin1,3; Zhang, Qiulong1,3; Hu, Na1,3; Suo, Yourui1; You, Jinmao1,2; Li, GL (reprint author), Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China.
2015-03-01
Source PublicationFOOD ANALYTICAL METHODS
Volume8Issue:3Pages:685-695
SubtypeArticle
AbstractA simple, sensitive and selective method based on one-step fluorescence labeling and ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) was developed for the determination of biogenic amines (BAs) in foodstuff samples by high-performance liquid chromatography (HPLC) with fluorescence detection (FLD). In this work, fluorescence probe 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) was applied to label BAs. What followed was the UA-DLLME procedure that was carried out using chloroform and acetone as extraction and disperser solvents, respectively. A response surface methodology (RSM) based on a Box-Behnken design (BBD) was employed to optimize the main parameters affecting the fluorescence labeling and DLLME efficiency. Under the optimal conditions, this method offered low limits of detection (LODs) of 1.1-7.8 ng/mL and limits of quantification (LOQs) of 3.5-26.1 ng/mL. Finally, the method was successfully used for the determination of trace BAs in real samples and exhibited powerful potential in the high-throughput sample screening.; A simple, sensitive and selective method based on one-step fluorescence labeling and ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) was developed for the determination of biogenic amines (BAs) in foodstuff samples by high-performance liquid chromatography (HPLC) with fluorescence detection (FLD). In this work, fluorescence probe 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) was applied to label BAs. What followed was the UA-DLLME procedure that was carried out using chloroform and acetone as extraction and disperser solvents, respectively. A response surface methodology (RSM) based on a Box-Behnken design (BBD) was employed to optimize the main parameters affecting the fluorescence labeling and DLLME efficiency. Under the optimal conditions, this method offered low limits of detection (LODs) of 1.1-7.8 ng/mL and limits of quantification (LOQs) of 3.5-26.1 ng/mL. Finally, the method was successfully used for the determination of trace BAs in real samples and exhibited powerful potential in the high-throughput sample screening.
KeywordBiogenic Amines Fluorescence Labeling Ultrasound-assisted Dispersive Liquid-liquid Microextraction Foodstuff Samples Box-behnken Design
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS KeywordCHROMATOGRAPHY-MASS SPECTROMETRY ; CAPILLARY-ELECTROPHORESIS ; ETHYL CHLOROFORMATE ; FISH PRODUCTS ; DERIVATIZATION ; IDENTIFICATION ; REAGENT ; WINES ; ACIDS ; MEAT
Indexed BySCI
Language英语
WOS Research AreaFood Science & Technology
WOS SubjectFood Science & Technology
WOS IDWOS:000349553900018
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/5451
Collection中国科学院西北高原生物研究所
Corresponding AuthorLi, GL (reprint author), Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China.
Affiliation1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China
2.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China
3.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China
4.Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China
Recommended Citation
GB/T 7714
Wu, Hongliang,Li, Guoliang,Liu, Shucheng,et al. Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology[J]. FOOD ANALYTICAL METHODS,2015,8(3):685-695.
APA Wu, Hongliang.,Li, Guoliang.,Liu, Shucheng.,Ji, Zhongyin.,Zhang, Qiulong.,...&Li, GL .(2015).Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology.FOOD ANALYTICAL METHODS,8(3),685-695.
MLA Wu, Hongliang,et al."Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology".FOOD ANALYTICAL METHODS 8.3(2015):685-695.
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