NWIPB OpenIR
Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology
Wu, Hongliang1,3; Li, Guoliang2,4; Liu, Shucheng4; Ji, Zhongyin1,3; Zhang, Qiulong1,3; Hu, Na1,3; Suo, Yourui1; You, Jinmao1,2; Li, GL (reprint author), Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China.
2015-03-01
发表期刊FOOD ANALYTICAL METHODS
卷号8期号:3页码:685-695
文章类型Article
摘要A simple, sensitive and selective method based on one-step fluorescence labeling and ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) was developed for the determination of biogenic amines (BAs) in foodstuff samples by high-performance liquid chromatography (HPLC) with fluorescence detection (FLD). In this work, fluorescence probe 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) was applied to label BAs. What followed was the UA-DLLME procedure that was carried out using chloroform and acetone as extraction and disperser solvents, respectively. A response surface methodology (RSM) based on a Box-Behnken design (BBD) was employed to optimize the main parameters affecting the fluorescence labeling and DLLME efficiency. Under the optimal conditions, this method offered low limits of detection (LODs) of 1.1-7.8 ng/mL and limits of quantification (LOQs) of 3.5-26.1 ng/mL. Finally, the method was successfully used for the determination of trace BAs in real samples and exhibited powerful potential in the high-throughput sample screening.; A simple, sensitive and selective method based on one-step fluorescence labeling and ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) was developed for the determination of biogenic amines (BAs) in foodstuff samples by high-performance liquid chromatography (HPLC) with fluorescence detection (FLD). In this work, fluorescence probe 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) was applied to label BAs. What followed was the UA-DLLME procedure that was carried out using chloroform and acetone as extraction and disperser solvents, respectively. A response surface methodology (RSM) based on a Box-Behnken design (BBD) was employed to optimize the main parameters affecting the fluorescence labeling and DLLME efficiency. Under the optimal conditions, this method offered low limits of detection (LODs) of 1.1-7.8 ng/mL and limits of quantification (LOQs) of 3.5-26.1 ng/mL. Finally, the method was successfully used for the determination of trace BAs in real samples and exhibited powerful potential in the high-throughput sample screening.
关键词Biogenic Amines Fluorescence Labeling Ultrasound-assisted Dispersive Liquid-liquid Microextraction Foodstuff Samples Box-behnken Design
WOS标题词Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]CHROMATOGRAPHY-MASS SPECTROMETRY ; CAPILLARY-ELECTROPHORESIS ; ETHYL CHLOROFORMATE ; FISH PRODUCTS ; DERIVATIZATION ; IDENTIFICATION ; REAGENT ; WINES ; ACIDS ; MEAT
收录类别SCI
语种英语
WOS研究方向Food Science & Technology
WOS类目Food Science & Technology
WOS记录号WOS:000349553900018
引用统计
被引频次:35[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/5451
专题中国科学院西北高原生物研究所
通讯作者Li, GL (reprint author), Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China.
作者单位1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China
2.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China
3.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China
4.Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China
推荐引用方式
GB/T 7714
Wu, Hongliang,Li, Guoliang,Liu, Shucheng,et al. Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology[J]. FOOD ANALYTICAL METHODS,2015,8(3):685-695.
APA Wu, Hongliang.,Li, Guoliang.,Liu, Shucheng.,Ji, Zhongyin.,Zhang, Qiulong.,...&Li, GL .(2015).Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology.FOOD ANALYTICAL METHODS,8(3),685-695.
MLA Wu, Hongliang,et al."Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology".FOOD ANALYTICAL METHODS 8.3(2015):685-695.
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