NWIPB OpenIR
A simple and sensitive HPLC method based on pre-column fluorescence labelling for multiple classes of plant growth regulator determination in food samples
Li, GL; Liu, SC; Sun, ZW; Xia, L; Chen, G; You, JM; Li, GL (reprint author), Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China.
2015
发表期刊FOOD CHEMISTRY
卷号170页码:123-130
摘要The determination of trace plant growth regulator (PGR) has received more and more attentions in the field of phytophysiology and food safety. But the simple and sensitive method for simultaneously analysing multiple classes of PGR remains poorly investigated. In this study, a new pre-column fluorescence labelling method using 2-(11H-benzo[a]carbazol-11-yl)-ethyl-4-methylbenzenesulfonate (BCETS) as the labelling reagent has been developed for simultaneous determination of seven PGRs (i.e., indole-3-acetic acid, 3-indolybutyric acid, 3-indolepropionic acid, jasmonic acid, gibberellin A(3), 1-naphthylacetic acid and 2-naphthaleneacetic acid) by HPLC with fluorescent detection (FLD). The proposed method offered the LOD of 0.34-0.73 ng/mL for seven PGRs, which were significantly lower than the reported methods. The crude extract without complex pre-treatments and purification was directly labelled by BCETS and analysed by HPLC-FLD, which facilitates the high-throughput sample screening. This method was proven to be inexpensive, simple, selective, sensitive, accurate and reliable for trace PGR determination. (C) 2014 Elsevier Ltd. All rights reserved.; The determination of trace plant growth regulator (PGR) has received more and more attentions in the field of phytophysiology and food safety. But the simple and sensitive method for simultaneously analysing multiple classes of PGR remains poorly investigated. In this study, a new pre-column fluorescence labelling method using 2-(11H-benzo[a]carbazol-11-yl)-ethyl-4-methylbenzenesulfonate (BCETS) as the labelling reagent has been developed for simultaneous determination of seven PGRs (i.e., indole-3-acetic acid, 3-indolybutyric acid, 3-indolepropionic acid, jasmonic acid, gibberellin A(3), 1-naphthylacetic acid and 2-naphthaleneacetic acid) by HPLC with fluorescent detection (FLD). The proposed method offered the LOD of 0.34-0.73 ng/mL for seven PGRs, which were significantly lower than the reported methods. The crude extract without complex pre-treatments and purification was directly labelled by BCETS and analysed by HPLC-FLD, which facilitates the high-throughput sample screening. This method was proven to be inexpensive, simple, selective, sensitive, accurate and reliable for trace PGR determination. (C) 2014 Elsevier Ltd. All rights reserved.
关键词Plant Growth Regulators Pre-column Fluorescence Labelling Hplc Food Samples
收录类别SCI
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/5454
专题中国科学院西北高原生物研究所
通讯作者Li, GL (reprint author), Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China.
推荐引用方式
GB/T 7714
Li, GL,Liu, SC,Sun, ZW,et al. A simple and sensitive HPLC method based on pre-column fluorescence labelling for multiple classes of plant growth regulator determination in food samples[J]. FOOD CHEMISTRY,2015,170:123-130.
APA Li, GL.,Liu, SC.,Sun, ZW.,Xia, L.,Chen, G.,...&Li, GL .(2015).A simple and sensitive HPLC method based on pre-column fluorescence labelling for multiple classes of plant growth regulator determination in food samples.FOOD CHEMISTRY,170,123-130.
MLA Li, GL,et al."A simple and sensitive HPLC method based on pre-column fluorescence labelling for multiple classes of plant growth regulator determination in food samples".FOOD CHEMISTRY 170(2015):123-130.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Li-2015-A simple and(1513KB) 开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, GL]的文章
[Liu, SC]的文章
[Sun, ZW]的文章
百度学术
百度学术中相似的文章
[Li, GL]的文章
[Liu, SC]的文章
[Sun, ZW]的文章
必应学术
必应学术中相似的文章
[Li, GL]的文章
[Liu, SC]的文章
[Sun, ZW]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Li-2015-A simple and sensiti.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。