NWIPB OpenIR
Preparative separation of a challenging anthocyanin from Lycium ruthenicum Murr. by two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography
Jin, Hongli1,2; Zhao, Jianqiang3; Zhou, Weijia1; Shen, Aijin1; Yang, Fan1; Liu, Yanfang1; Guo, Zhimou1; Zhang, Xiuli1; Tao, Yanduo3; Peng, Xiaojun2; Liang, Xinmiao1; Liu, YF (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China.
2015
发表期刊RSC ADVANCES
卷号5期号:76页码:62134-62141
文章类型Article
摘要The preparative separation of anthocyanins by HPLC often suffers from insufficient separation selectivity. In this work, a two-dimensional liquid (LC-LC) method was established to efficiently purify a challenging anthocyanin in Lycium ruthenicum Murray. Reversed phase liquid chromatography (RPLC) was used in the first-dimension preparation to fractionate the sample for its high separation efficiency. After the optimization of second-dimension methods, hydrophilic interaction chromatography (HILIC) was applied to further isolate the anthocyanin for the good orthogonality to RPLC. To improve HILIC separation for anthocyanins, stationary phases and mobile phases were investigated systematically. A satisfactory result was obtained on a zwitterionic Click XIon column with 1% phosphoric acid as an acidic additive. Using the above method, the anthocyanin and three new alkaloids were isolated from L. ruthenicum for the first time. This RPLC/HILIC method solved the coelution problem of anthocyanin and basic non-anthocyanins in one-dimensional HPLC, benefiting from the significantly improved separation resolution.; The preparative separation of anthocyanins by HPLC often suffers from insufficient separation selectivity. In this work, a two-dimensional liquid (LC-LC) method was established to efficiently purify a challenging anthocyanin in Lycium ruthenicum Murray. Reversed phase liquid chromatography (RPLC) was used in the first-dimension preparation to fractionate the sample for its high separation efficiency. After the optimization of second-dimension methods, hydrophilic interaction chromatography (HILIC) was applied to further isolate the anthocyanin for the good orthogonality to RPLC. To improve HILIC separation for anthocyanins, stationary phases and mobile phases were investigated systematically. A satisfactory result was obtained on a zwitterionic Click XIon column with 1% phosphoric acid as an acidic additive. Using the above method, the anthocyanin and three new alkaloids were isolated from L. ruthenicum for the first time. This RPLC/HILIC method solved the coelution problem of anthocyanin and basic non-anthocyanins in one-dimensional HPLC, benefiting from the significantly improved separation resolution.
WOS标题词Science & Technology ; Physical Sciences
关键词[WOS]HYDROPHILIC-INTERACTION CHROMATOGRAPHY ; CHARGED C18 COLUMN ; STATIONARY-PHASE ; MASS-SPECTROMETRY ; POLAR COMPOUNDS ; PURIFICATION ; BUFADIENOLIDES ; PEPTIDES ; EXTRACTS ; SKIN
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Multidisciplinary
WOS记录号WOS:000358353100077
引用统计
被引频次:34[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/5472
专题中国科学院西北高原生物研究所
通讯作者Liu, YF (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China.
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China
推荐引用方式
GB/T 7714
Jin, Hongli,Zhao, Jianqiang,Zhou, Weijia,et al. Preparative separation of a challenging anthocyanin from Lycium ruthenicum Murr. by two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography[J]. RSC ADVANCES,2015,5(76):62134-62141.
APA Jin, Hongli.,Zhao, Jianqiang.,Zhou, Weijia.,Shen, Aijin.,Yang, Fan.,...&Liu, YF .(2015).Preparative separation of a challenging anthocyanin from Lycium ruthenicum Murr. by two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography.RSC ADVANCES,5(76),62134-62141.
MLA Jin, Hongli,et al."Preparative separation of a challenging anthocyanin from Lycium ruthenicum Murr. by two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography".RSC ADVANCES 5.76(2015):62134-62141.
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