Isolation of microsatellite loci for Rhodiola alsia (Crassulaceae), an important ethno-medicinal herb endemic to the Qinghai-Tibetan plateau

NWIPB OpenIR

	Isolation of microsatellite loci for Rhodiola alsia (Crassulaceae), an important ethno-medicinal herb endemic to the Qinghai-Tibetan plateau
	Zhang, F. Q.1,2; Lei, S. Y.2; Gao, Q. B.2; Khan, G.2; Xing, R.2; Yang, H. L.2; Chen, S. L.2; Chen, SL (reprint author), Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Inst Plateau Biol, Xining, Peoples R China.
	2015
发表期刊	GENETICS AND MOLECULAR RESEARCH
卷号	14 期号:2 页码:5266-5269
文章类型	Article
摘要	Rhodiola alsia, which has been used widely in traditional Chinese medicine for a considerable time, grows on moist habitats at high altitude near the snow line. Microsatellite loci were developed for R. alsia to investigate its population genetics. In total, 17 polymorphic microsatellites were developed based on ESTs from the Illumina HiSeqTM 2000 platform. The microsatellite loci were checked for variability using 80 individuals of R. alsia sampled from four locations on the Qinghai-Tibet Plateau. The total number of alleles per locus ranged from 10 to 20, and the observed heterozygosity ranged from 0.000 to 1.000. The null allele frequency ranged from 0.000 to 0.324. These microsatellites are expected to be helpful in future studies of population genetics in R. alsia and related species.; Rhodiola alsia, which has been used widely in traditional Chinese medicine for a considerable time, grows on moist habitats at high altitude near the snow line. Microsatellite loci were developed for R. alsia to investigate its population genetics. In total, 17 polymorphic microsatellites were developed based on ESTs from the Illumina HiSeqTM 2000 platform. The microsatellite loci were checked for variability using 80 individuals of R. alsia sampled from four locations on the Qinghai-Tibet Plateau. The total number of alleles per locus ranged from 10 to 20, and the observed heterozygosity ranged from 0.000 to 1.000. The null allele frequency ranged from 0.000 to 0.324. These microsatellites are expected to be helpful in future studies of population genetics in R. alsia and related species.
关键词	Rhodiola Alsia Simple Sequence Repeat Population Genetics Qinghai-tibet Plateau
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	DNA
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号	WOS:000357183700249
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5475
专题	中国科学院西北高原生物研究所
通讯作者	Chen, SL (reprint author), Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Inst Plateau Biol, Xining, Peoples R China.
作者单位	1.Southwest Univ, Sch Life Sci, Minist Educ, Key Lab Eco Environm Three Gorges Reservoir Reg, Chongqing, Peoples R China 2.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Inst Plateau Biol, Xining, Peoples R China
推荐引用方式 GB/T 7714	Zhang, F. Q.,Lei, S. Y.,Gao, Q. B.,et al. Isolation of microsatellite loci for Rhodiola alsia (Crassulaceae), an important ethno-medicinal herb endemic to the Qinghai-Tibetan plateau[J]. GENETICS AND MOLECULAR RESEARCH,2015,14(2):5266-5269.
APA	Zhang, F. Q..,Lei, S. Y..,Gao, Q. B..,Khan, G..,Xing, R..,...&Chen, SL .(2015).Isolation of microsatellite loci for Rhodiola alsia (Crassulaceae), an important ethno-medicinal herb endemic to the Qinghai-Tibetan plateau.GENETICS AND MOLECULAR RESEARCH,14(2),5266-5269.
MLA	Zhang, F. Q.,et al."Isolation of microsatellite loci for Rhodiola alsia (Crassulaceae), an important ethno-medicinal herb endemic to the Qinghai-Tibetan plateau".GENETICS AND MOLECULAR RESEARCH 14.2(2015):5266-5269.

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