Determination of multiple phytohormones in fruits by high-performance liquid chromatography with fluorescence detection using dispersive liquid-liquid microextraction followed by precolumn fluorescent labeling

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	Determination of multiple phytohormones in fruits by high-performance liquid chromatography with fluorescence detection using dispersive liquid-liquid microextraction followed by precolumn fluorescent labeling
	Li, GL; Lu, SM; Wu, HL; Chen, G; Liu, SC; Kong, XJ; Kong, WH; You, JM; Li, GL (reprint author), Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Shandong, Peoples R China.
	2015
发表期刊	JOURNAL OF SEPARATION SCIENCE
卷号	38 期号:2 页码:187-196
摘要	Plant hormone determination in food matrices has attracted more and more attention because of their potential risks to human health. However, analytical methods for the analysis of multiple plant hormones remain poorly investigated. In the present study, a convenient, selective, and ultrasensitive high-performance liquid chromatography method for the simultaneous determination of multiple classes of plant hormones has been developed successfully using dispersive liquid-liquid microextraction followed by precolumn fluorescent labeling. Eight plant hormones in fruits including jasmonic acid, 12-oxo-phytodienoic acid, indole-3-acetic acid, 3-indolybutyric acid, 3-indolepropionic acid, gibberellin A(3), 1-naphthylacetic acid, and 2-naphthaleneacetic acid were analyzed by this method. The conditions employed for dispersive liquid-liquid microextraction were optimized systematically. The linearity for all plant hormones was found to be >0.9993 (R-2 values). This method offered low detection limits of 0.19-0.44 ng/mL (at a signal-to-noise ratio of 3), and method accuracies were in the range of 92.32-103.10%. The proposed method was applied to determine plant hormones in five kinds of food samples, and this method can achieve a short analysis time, low threshold levels of detection, and a high specificity for the analysis of targeted plant hormones present at trace level concentrations in complex matrices.; Plant hormone determination in food matrices has attracted more and more attention because of their potential risks to human health. However, analytical methods for the analysis of multiple plant hormones remain poorly investigated. In the present study, a convenient, selective, and ultrasensitive high-performance liquid chromatography method for the simultaneous determination of multiple classes of plant hormones has been developed successfully using dispersive liquid-liquid microextraction followed by precolumn fluorescent labeling. Eight plant hormones in fruits including jasmonic acid, 12-oxo-phytodienoic acid, indole-3-acetic acid, 3-indolybutyric acid, 3-indolepropionic acid, gibberellin A(3), 1-naphthylacetic acid, and 2-naphthaleneacetic acid were analyzed by this method. The conditions employed for dispersive liquid-liquid microextraction were optimized systematically. The linearity for all plant hormones was found to be >0.9993 (R-2 values). This method offered low detection limits of 0.19-0.44 ng/mL (at a signal-to-noise ratio of 3), and method accuracies were in the range of 92.32-103.10%. The proposed method was applied to determine plant hormones in five kinds of food samples, and this method can achieve a short analysis time, low threshold levels of detection, and a high specificity for the analysis of targeted plant hormones present at trace level concentrations in complex matrices.
关键词	Fluorescent Labeling Fruits High-performance Liquid Chromatography Plant Hormones
收录类别	SCI
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5481
专题	中国科学院西北高原生物研究所
通讯作者	Li, GL (reprint author), Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Shandong, Peoples R China.
推荐引用方式 GB/T 7714	Li, GL,Lu, SM,Wu, HL,et al. Determination of multiple phytohormones in fruits by high-performance liquid chromatography with fluorescence detection using dispersive liquid-liquid microextraction followed by precolumn fluorescent labeling[J]. JOURNAL OF SEPARATION SCIENCE,2015,38(2):187-196.
APA	Li, GL.,Lu, SM.,Wu, HL.,Chen, G.,Liu, SC.,...&Li, GL .(2015).Determination of multiple phytohormones in fruits by high-performance liquid chromatography with fluorescence detection using dispersive liquid-liquid microextraction followed by precolumn fluorescent labeling.JOURNAL OF SEPARATION SCIENCE,38(2),187-196.
MLA	Li, GL,et al."Determination of multiple phytohormones in fruits by high-performance liquid chromatography with fluorescence detection using dispersive liquid-liquid microextraction followed by precolumn fluorescent labeling".JOURNAL OF SEPARATION SCIENCE 38.2(2015):187-196.

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