Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Rapid Separation of Three Proanthocyanidin Dimers from Iris lactea Pall. var. Chinensis (Fisch.) Koidz by High-Speed Counter-Current Chromatography With Continuous Sample Load and Double-Pump Balancing Mode | |
Lv, Huanhuan1,2; Yuan, Zhenzhen1,2; Wang, Xiaoyan1; Wang, Zhenhua1; Suo, Yourui1; Wang, Honglun1 | |
2015-11-01 | |
发表期刊 | PHYTOCHEMICAL ANALYSIS |
卷号 | 26期号:6页码:444-453 |
文章类型 | Article |
摘要 | Introduction - The dried seeds of Iris lactea have been used in traditional Chinese medicine. Previous studies have been focused on irisquinones while other chemical components are rarely reported. Objective - To establish an efficient high-speed counter-current chromatography (HSCCC) separation method with continuous sample load (CSL) and double-pump balancing (DPB) mode to isolate proanthocyanidins from I. lactea. Methods - Firstly, an ethyl acetate extract of I. lacteawas pre-fractionated by silica column chromatography for the enrichment of proanthocyanidins. Secondly, the enriched proanthocyanidins sample ( EPS) was further fractionated by HSCCC with a two-phase solvent system ethyl acetate: n- butanol: water ( 9: 1: 10, v/ v/ v) using DPB mode. The flow rate of the two phases was 2.2mL/ min, the revolution speed was 900 rpm, the separation temperature was 30 degrees C and the detection wavelength was 280nm. Finally, the structures of the three isolated proanthocyanidins were elucidated by spectroscopic methods and compared with published data. Results - Under the optimized conditions, 600mg of the EPS with six continuous injections ( 100mg/ time) was fractionated, yielding 57mg of prodelphinidin B3, 198mg of procyanidin B3, and 162mg of procyanidin B1, at purities of 97.2%, 98.1% and 97.3%, respectively. Conclusions - The HSCCC separation method with CSL and DPB proved to be rapid, convenient and economical, constituting an efficient strategy for the isolation of proanthocyanidins. Copyright (c) 2015 John Wiley & Sons, Ltd.; Introduction - The dried seeds of Iris lactea have been used in traditional Chinese medicine. Previous studies have been focused on irisquinones while other chemical components are rarely reported. Objective - To establish an efficient high-speed counter-current chromatography (HSCCC) separation method with continuous sample load (CSL) and double-pump balancing (DPB) mode to isolate proanthocyanidins from I. lactea. Methods - Firstly, an ethyl acetate extract of I. lacteawas pre-fractionated by silica column chromatography for the enrichment of proanthocyanidins. Secondly, the enriched proanthocyanidins sample ( EPS) was further fractionated by HSCCC with a two-phase solvent system ethyl acetate: n- butanol: water ( 9: 1: 10, v/ v/ v) using DPB mode. The flow rate of the two phases was 2.2mL/ min, the revolution speed was 900 rpm, the separation temperature was 30 degrees C and the detection wavelength was 280nm. Finally, the structures of the three isolated proanthocyanidins were elucidated by spectroscopic methods and compared with published data. Results - Under the optimized conditions, 600mg of the EPS with six continuous injections ( 100mg/ time) was fractionated, yielding 57mg of prodelphinidin B3, 198mg of procyanidin B3, and 162mg of procyanidin B1, at purities of 97.2%, 98.1% and 97.3%, respectively. Conclusions - The HSCCC separation method with CSL and DPB proved to be rapid, convenient and economical, constituting an efficient strategy for the isolation of proanthocyanidins. Copyright (c) 2015 John Wiley & Sons, Ltd. |
关键词 | Iris Lactea Pall Var Chinensis (Fisch.) Koidz Proanthocyanidins Hsccc Continuous Sample Load Double-pump Balancing Mode |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
DOI | 10.1002/pca.2579 |
关键词[WOS] | PREPARATIVE ISOLATION ; PURIFICATION ; PROCYANIDINS ; HPLC ; BIOTRANSFORMATION ; IDENTIFICATION ; FLAVAN-3-OLS ; EXTRACTION ; COMPONENTS ; LEAVES |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | National Science Foundation of China(31470426 ; Open Topic of Key Laboratory of Tibetan Medicine Research, CAS(2014-TMR-03) ; Youth Innovation Promotion Association, CAS ; Key Programme of "The Dawn of West China" Talent Foundation of CAS ; 31300292) |
WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry |
WOS类目 | Biochemical Research Methods ; Plant Sciences ; Chemistry, Analytical |
WOS记录号 | WOS:000363332400008 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/5491 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Lv, Huanhuan,Yuan, Zhenzhen,Wang, Xiaoyan,et al. Rapid Separation of Three Proanthocyanidin Dimers from Iris lactea Pall. var. Chinensis (Fisch.) Koidz by High-Speed Counter-Current Chromatography With Continuous Sample Load and Double-Pump Balancing Mode[J]. PHYTOCHEMICAL ANALYSIS,2015,26(6):444-453. |
APA | Lv, Huanhuan,Yuan, Zhenzhen,Wang, Xiaoyan,Wang, Zhenhua,Suo, Yourui,&Wang, Honglun.(2015).Rapid Separation of Three Proanthocyanidin Dimers from Iris lactea Pall. var. Chinensis (Fisch.) Koidz by High-Speed Counter-Current Chromatography With Continuous Sample Load and Double-Pump Balancing Mode.PHYTOCHEMICAL ANALYSIS,26(6),444-453. |
MLA | Lv, Huanhuan,et al."Rapid Separation of Three Proanthocyanidin Dimers from Iris lactea Pall. var. Chinensis (Fisch.) Koidz by High-Speed Counter-Current Chromatography With Continuous Sample Load and Double-Pump Balancing Mode".PHYTOCHEMICAL ANALYSIS 26.6(2015):444-453. |
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