Sensitive Determination of Amantadine in Microdialysis Samples from Rat Plasma by HPLC with Fluorescence Detection

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	Sensitive Determination of Amantadine in Microdialysis Samples from Rat Plasma by HPLC with Fluorescence Detection
	Wang, FF; Zhang, SJ; Sheng, CC; Zhao, XE; You, JM
	2015
发表期刊	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
卷号	38 期号:17 页码:1622-1628
摘要	A new and sensitive high-performance liquid chromatography (HPLC) method with 10-methyl-acridone-2-sulfonyl chloride (MASC) as pre-column fluorescence labeling reagent was developed for the determination of amantadine in microdialysis samples from rat plasma. The HPLC sensitivity of amantadine was greatly enhanced by introducing MASC with large conjugated structure and excellent fluorescence property into the amantadine molecule. The limit of detection was 6.8ngmL(-1) and only 30L sample was needed for the pharmacokinetic study. The linearity range was 25-500ngmL(-1) with correlation coefficients of 0.9989. The proposed method was successfully applied to the determination of amantadine in microdialysis samples from rat plasma. It also shows powerful potential for the pharmacokinetic study of compounds with amino group.; A new and sensitive high-performance liquid chromatography (HPLC) method with 10-methyl-acridone-2-sulfonyl chloride (MASC) as pre-column fluorescence labeling reagent was developed for the determination of amantadine in microdialysis samples from rat plasma. The HPLC sensitivity of amantadine was greatly enhanced by introducing MASC with large conjugated structure and excellent fluorescence property into the amantadine molecule. The limit of detection was 6.8ngmL(-1) and only 30L sample was needed for the pharmacokinetic study. The linearity range was 25-500ngmL(-1) with correlation coefficients of 0.9989. The proposed method was successfully applied to the determination of amantadine in microdialysis samples from rat plasma. It also shows powerful potential for the pharmacokinetic study of compounds with amino group.
关键词	Amantadine Fluorescence Derivatization Hplc Microdialysis
收录类别	SCI
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5493
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, FF,Zhang, SJ,Sheng, CC,et al. Sensitive Determination of Amantadine in Microdialysis Samples from Rat Plasma by HPLC with Fluorescence Detection[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2015,38(17):1622-1628.
APA	Wang, FF,Zhang, SJ,Sheng, CC,Zhao, XE,&You, JM.(2015).Sensitive Determination of Amantadine in Microdialysis Samples from Rat Plasma by HPLC with Fluorescence Detection.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,38(17),1622-1628.
MLA	Wang, FF,et al."Sensitive Determination of Amantadine in Microdialysis Samples from Rat Plasma by HPLC with Fluorescence Detection".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 38.17(2015):1622-1628.