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Transcriptome-wide identification, molecular evolution and expression analysis of Toll-like receptor family in a Tibet fish, Gymnocypris przewalskii
Tong, Chao1,2,3; Lin, Yaqiu4; Zhang, Cunfang1,2; Shi, Jianquan5; Qi, Hongfang5; Zhao, Kai1,2
2015-10-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume46Issue:2Pages:334-345
SubtypeArticle
AbstractToll-like receptors (TLR) are key components of innate immunity that play significant roles in immune defense against pathogens invasion. Recent frequent outbreaks of the "white spot disease" caused by parasitic infection in farmed Tibetan fishes had resulted in great economic losses. However, to our knowledge, the roles of TLRs in mediating immune response to parasitic infection in Tibetan fishes remain to be determined. Here, we performed data-mining on a widely-farmed Tibetan fish (Gymnocypris przewalskii or Gp) transcriptome to determine the genetic variation and expression pattern of TLRs. We totally obtained 14 GpTLRs and identified 5 with a complete coding sequence. Phylogenetic analysis verified their identities and supported the classification of TLRs into six families as in other vertebrates. The TLR family motifs, such as leucine rich repeat (LRR) and Toll/interleukin (IL)-1 receptor (TIR) domain, are conserved in GpTLR1-5. Selective pressure test demonstrated that all known GpTLRs are under purifying selection, except GpTLR4 underwent positive selection. Further, site model analysis suggested that 11 positively selected sites are found in LRR domain of GpTLR4. Three positively selected sites are located on outside surface of TLR4 3D structure, indicating that function of GpTLR4 may be affected. Tissue specific expression analysis showed all GpTLRs are present in gill, head-kidney and spleen but the relative abundance varied among tissues. In response to parasite Ichthyophthirius multifiliis infection, 5 GpTLR (GpTLR1, -2, -4, -9 and -20) expressions were induced. Intriguingly, GpTLR4 was significantly up-regulated in gills, while GpTLR19 and GpTLR21 unexpectedly showed no any change. In summary, these results revealed the first genomic resources of TLR family and several parasitic infection responsive TLRs in Tibetan fish. These findings provide key information for future studies aiming to understand the molecular mechanisms underlying the immune response to pathogen invasion in Tibetan fishes. (C) 2015 Elsevier Ltd. All rights reserved.; Toll-like receptors (TLR) are key components of innate immunity that play significant roles in immune defense against pathogens invasion. Recent frequent outbreaks of the "white spot disease" caused by parasitic infection in farmed Tibetan fishes had resulted in great economic losses. However, to our knowledge, the roles of TLRs in mediating immune response to parasitic infection in Tibetan fishes remain to be determined. Here, we performed data-mining on a widely-farmed Tibetan fish (Gymnocypris przewalskii or Gp) transcriptome to determine the genetic variation and expression pattern of TLRs. We totally obtained 14 GpTLRs and identified 5 with a complete coding sequence. Phylogenetic analysis verified their identities and supported the classification of TLRs into six families as in other vertebrates. The TLR family motifs, such as leucine rich repeat (LRR) and Toll/interleukin (IL)-1 receptor (TIR) domain, are conserved in GpTLR1-5. Selective pressure test demonstrated that all known GpTLRs are under purifying selection, except GpTLR4 underwent positive selection. Further, site model analysis suggested that 11 positively selected sites are found in LRR domain of GpTLR4. Three positively selected sites are located on outside surface of TLR4 3D structure, indicating that function of GpTLR4 may be affected. Tissue specific expression analysis showed all GpTLRs are present in gill, head-kidney and spleen but the relative abundance varied among tissues. In response to parasite Ichthyophthirius multifiliis infection, 5 GpTLR (GpTLR1, -2, -4, -9 and -20) expressions were induced. Intriguingly, GpTLR4 was significantly up-regulated in gills, while GpTLR19 and GpTLR21 unexpectedly showed no any change. In summary, these results revealed the first genomic resources of TLR family and several parasitic infection responsive TLRs in Tibetan fish. These findings provide key information for future studies aiming to understand the molecular mechanisms underlying the immune response to pathogen invasion in Tibetan fishes. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordGymnocypris Przewalskii Toll-like Receptor Transcriptome Innate Immune Ichthyophthirius Multifiliis Infection
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.fsi.2015.06.023
WOS KeywordNF-KAPPA-B ; POSITIVE SELECTION ; ICHTHYOPHTHIRIUS-MULTIFILIIS ; PROINFLAMMATORY RESPONSES ; PLASMODIUM-FALCIPARUM ; ICTALURUS-PUNCTATUS ; MAXIMUM-LIKELIHOOD ; TRYPANOSOMA-CRUZI ; CHANNEL CATFISH ; INNATE IMMUNITY
Indexed BySCI
Language英语
Funding OrganizationChinese Academy of Sciences(KSCX2-YW-N-101 ; National Natural Science Foundation of China(30970341 ; West Light Foundation from the Chinese Academy of Sciences ; KSCX2-1W-N-004) ; 31172070)
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000363071200021
Citation statistics
Cited Times:62[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/5498
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Lab Plateau Fish Evolutionary & Funct Genom, Xining 810001, Qinghai, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Southwest Univ Nationalities, Coll Life Sci & Technol, Chengdu 610041, Peoples R China
5.Rescues Ctr Qinghai Lake Naked Carp, Xining 810016, Peoples R China
Recommended Citation
GB/T 7714
Tong, Chao,Lin, Yaqiu,Zhang, Cunfang,et al. Transcriptome-wide identification, molecular evolution and expression analysis of Toll-like receptor family in a Tibet fish, Gymnocypris przewalskii[J]. FISH & SHELLFISH IMMUNOLOGY,2015,46(2):334-345.
APA Tong, Chao,Lin, Yaqiu,Zhang, Cunfang,Shi, Jianquan,Qi, Hongfang,&Zhao, Kai.(2015).Transcriptome-wide identification, molecular evolution and expression analysis of Toll-like receptor family in a Tibet fish, Gymnocypris przewalskii.FISH & SHELLFISH IMMUNOLOGY,46(2),334-345.
MLA Tong, Chao,et al."Transcriptome-wide identification, molecular evolution and expression analysis of Toll-like receptor family in a Tibet fish, Gymnocypris przewalskii".FISH & SHELLFISH IMMUNOLOGY 46.2(2015):334-345.
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