Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Transcriptome-wide identification, molecular evolution and expression analysis of Toll-like receptor family in a Tibet fish, Gymnocypris przewalskii | |
| Tong, Chao1,2,3; Lin, Yaqiu4; Zhang, Cunfang1,2; Shi, Jianquan5; Qi, Hongfang5; Zhao, Kai1,2 | |
| 2015-10-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
 |
| 卷号 | 46期号:2页码:334-345 |
| 文章类型 | Article |
| 摘要 | Toll-like receptors (TLR) are key components of innate immunity that play significant roles in immune defense against pathogens invasion. Recent frequent outbreaks of the "white spot disease" caused by parasitic infection in farmed Tibetan fishes had resulted in great economic losses. However, to our knowledge, the roles of TLRs in mediating immune response to parasitic infection in Tibetan fishes remain to be determined. Here, we performed data-mining on a widely-farmed Tibetan fish (Gymnocypris przewalskii or Gp) transcriptome to determine the genetic variation and expression pattern of TLRs. We totally obtained 14 GpTLRs and identified 5 with a complete coding sequence. Phylogenetic analysis verified their identities and supported the classification of TLRs into six families as in other vertebrates. The TLR family motifs, such as leucine rich repeat (LRR) and Toll/interleukin (IL)-1 receptor (TIR) domain, are conserved in GpTLR1-5. Selective pressure test demonstrated that all known GpTLRs are under purifying selection, except GpTLR4 underwent positive selection. Further, site model analysis suggested that 11 positively selected sites are found in LRR domain of GpTLR4. Three positively selected sites are located on outside surface of TLR4 3D structure, indicating that function of GpTLR4 may be affected. Tissue specific expression analysis showed all GpTLRs are present in gill, head-kidney and spleen but the relative abundance varied among tissues. In response to parasite Ichthyophthirius multifiliis infection, 5 GpTLR (GpTLR1, -2, -4, -9 and -20) expressions were induced. Intriguingly, GpTLR4 was significantly up-regulated in gills, while GpTLR19 and GpTLR21 unexpectedly showed no any change. In summary, these results revealed the first genomic resources of TLR family and several parasitic infection responsive TLRs in Tibetan fish. These findings provide key information for future studies aiming to understand the molecular mechanisms underlying the immune response to pathogen invasion in Tibetan fishes. (C) 2015 Elsevier Ltd. All rights reserved.; Toll-like receptors (TLR) are key components of innate immunity that play significant roles in immune defense against pathogens invasion. Recent frequent outbreaks of the "white spot disease" caused by parasitic infection in farmed Tibetan fishes had resulted in great economic losses. However, to our knowledge, the roles of TLRs in mediating immune response to parasitic infection in Tibetan fishes remain to be determined. Here, we performed data-mining on a widely-farmed Tibetan fish (Gymnocypris przewalskii or Gp) transcriptome to determine the genetic variation and expression pattern of TLRs. We totally obtained 14 GpTLRs and identified 5 with a complete coding sequence. Phylogenetic analysis verified their identities and supported the classification of TLRs into six families as in other vertebrates. The TLR family motifs, such as leucine rich repeat (LRR) and Toll/interleukin (IL)-1 receptor (TIR) domain, are conserved in GpTLR1-5. Selective pressure test demonstrated that all known GpTLRs are under purifying selection, except GpTLR4 underwent positive selection. Further, site model analysis suggested that 11 positively selected sites are found in LRR domain of GpTLR4. Three positively selected sites are located on outside surface of TLR4 3D structure, indicating that function of GpTLR4 may be affected. Tissue specific expression analysis showed all GpTLRs are present in gill, head-kidney and spleen but the relative abundance varied among tissues. In response to parasite Ichthyophthirius multifiliis infection, 5 GpTLR (GpTLR1, -2, -4, -9 and -20) expressions were induced. Intriguingly, GpTLR4 was significantly up-regulated in gills, while GpTLR19 and GpTLR21 unexpectedly showed no any change. In summary, these results revealed the first genomic resources of TLR family and several parasitic infection responsive TLRs in Tibetan fish. These findings provide key information for future studies aiming to understand the molecular mechanisms underlying the immune response to pathogen invasion in Tibetan fishes. (C) 2015 Elsevier Ltd. All rights reserved. |
| 关键词 | Gymnocypris Przewalskii Toll-like Receptor Transcriptome Innate Immune Ichthyophthirius Multifiliis Infection |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1016/j.fsi.2015.06.023 |
| 关键词[WOS] | NF-KAPPA-B ; POSITIVE SELECTION ; ICHTHYOPHTHIRIUS-MULTIFILIIS ; PROINFLAMMATORY RESPONSES ; PLASMODIUM-FALCIPARUM ; ICTALURUS-PUNCTATUS ; MAXIMUM-LIKELIHOOD ; TRYPANOSOMA-CRUZI ; CHANNEL CATFISH ; INNATE IMMUNITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | Chinese Academy of Sciences(KSCX2-YW-N-101 ; National Natural Science Foundation of China(30970341 ; West Light Foundation from the Chinese Academy of Sciences ; KSCX2-1W-N-004) ; 31172070) |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000363071200021 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/5498 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Lab Plateau Fish Evolutionary & Funct Genom, Xining 810001, Qinghai, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Southwest Univ Nationalities, Coll Life Sci & Technol, Chengdu 610041, Peoples R China 5.Rescues Ctr Qinghai Lake Naked Carp, Xining 810016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tong, Chao,Lin, Yaqiu,Zhang, Cunfang,et al. Transcriptome-wide identification, molecular evolution and expression analysis of Toll-like receptor family in a Tibet fish, Gymnocypris przewalskii[J]. FISH & SHELLFISH IMMUNOLOGY,2015,46(2):334-345. |
| APA | Tong, Chao,Lin, Yaqiu,Zhang, Cunfang,Shi, Jianquan,Qi, Hongfang,&Zhao, Kai.(2015).Transcriptome-wide identification, molecular evolution and expression analysis of Toll-like receptor family in a Tibet fish, Gymnocypris przewalskii.FISH & SHELLFISH IMMUNOLOGY,46(2),334-345. |
| MLA | Tong, Chao,et al."Transcriptome-wide identification, molecular evolution and expression analysis of Toll-like receptor family in a Tibet fish, Gymnocypris przewalskii".FISH & SHELLFISH IMMUNOLOGY 46.2(2015):334-345. |
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