Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Accurate Analysis and Evaluation of Acidic Plant Growth Regulators in Transgenic and Nontransgenic Edible Oils with Facile Microwave-Assisted Extraction-Derivatization | |
Liu, Mengge1,2; Chen, Guang1,2,3; Guo, Hailong1,2; Fan, Baoki4; Liu, Jianjun1,2; Fu, Qiang5; Li, Xiu1,2; Lu, Xiaomin1,2; Zhao, Xianen1,2; Li, Guoliang1,2; Sun, Zhiwei1,2; Xia, Lian1,2; Zhu, Shuyun1,2; Yang, Daoshan1,2; Cao, Ziping1,2; Wang, Hua1,2; Suo, Yourui3; You, Jinmao1,2,3 | |
2015-09-16 | |
发表期刊 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY |
卷号 | 63期号:36页码:8058-8067 |
文章类型 | Article |
摘要 | Determination of plant growth regulators (PGRs) in a signal transduction system (STS) is significant for transgenic food safety, but may be challenged by poor accuracy and analyte instability. In this work, a microwave-assisted extraction-derivatization (MAED) method is developed for six acidic PGRs in oil samples, allowing an efficient (<1.5 h) and facile (one step) pretreatment. Accuracies are greatly improved, particularly for gibberellin A(3) (-2.72 to -0.65%) as compared with those reported (-22 to -2%). Excellent selectivity and quite low detection limits (0.37-1.36 ng mL(-1)) are enabled by fluorescence detection-mass spectrum monitoring. Results show the significant differences in acidic PGRs between transgenic and nontransgenic oils, particularly 1-naphthaleneacetic acid (1-NAA), implying the PGRs induced variations of components and genes. This study provides, for the first time, an accurate and efficient determination for labile PGRs involved in STS and a promising concept for objectively evaluating the safety of transgenic foods.; Determination of plant growth regulators (PGRs) in a signal transduction system (STS) is significant for transgenic food safety, but may be challenged by poor accuracy and analyte instability. In this work, a microwave-assisted extraction-derivatization (MAED) method is developed for six acidic PGRs in oil samples, allowing an efficient (<1.5 h) and facile (one step) pretreatment. Accuracies are greatly improved, particularly for gibberellin A(3) (-2.72 to -0.65%) as compared with those reported (-22 to -2%). Excellent selectivity and quite low detection limits (0.37-1.36 ng mL(-1)) are enabled by fluorescence detection-mass spectrum monitoring. Results show the significant differences in acidic PGRs between transgenic and nontransgenic oils, particularly 1-naphthaleneacetic acid (1-NAA), implying the PGRs induced variations of components and genes. This study provides, for the first time, an accurate and efficient determination for labile PGRs involved in STS and a promising concept for objectively evaluating the safety of transgenic foods. |
关键词 | Plant Growth Regulators (Pgrs) Microwave-assisted Extraction-derivatization (Maed) Transgenic Food Signal Transduction System (Sts) |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; INDUCED FLUORESCENCE DETECTION ; CHEMICAL DERIVATIZATION ; GENE-EXPRESSION ; ALPHA-AMYLASE ; ABSCISIC-ACID ; CAPILLARY-ELECTROPHORESIS ; MAJOR PHYTOHORMONES ; JASMONIC ACID |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Agriculture ; Chemistry ; Food Science & Technology |
WOS类目 | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
WOS记录号 | WOS:000361502000021 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/5507 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China 4.Hubei Univ Sci & Technol, Xianning 437100, Peoples R China 5.Qinghai Normal Univ, Xining 810008, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Mengge,Chen, Guang,Guo, Hailong,et al. Accurate Analysis and Evaluation of Acidic Plant Growth Regulators in Transgenic and Nontransgenic Edible Oils with Facile Microwave-Assisted Extraction-Derivatization[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2015,63(36):8058-8067. |
APA | Liu, Mengge.,Chen, Guang.,Guo, Hailong.,Fan, Baoki.,Liu, Jianjun.,...&You, Jinmao.(2015).Accurate Analysis and Evaluation of Acidic Plant Growth Regulators in Transgenic and Nontransgenic Edible Oils with Facile Microwave-Assisted Extraction-Derivatization.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,63(36),8058-8067. |
MLA | Liu, Mengge,et al."Accurate Analysis and Evaluation of Acidic Plant Growth Regulators in Transgenic and Nontransgenic Edible Oils with Facile Microwave-Assisted Extraction-Derivatization".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 63.36(2015):8058-8067. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论